Data analysis If we call each step on the x-axis one volume of base, how many volumes of base must be added to change the pH from 4 to 6?
To determine: The volumes of the base need to add on the x-axis to change the pH from 4 to 6.
Introduction: Buffer solution is an aqueous solution that contains a solution of a weak acid and connected base. All living cells and fluid present in the multicellular organism contain a neutral pH.
Explanation of Solution
The basicity of the solution depends upon the ion concentration of H+ ions and OH- ions. Every basic solution has a pH value above 7. The range of basic solution in pH scale lies above 7 till 14. A basic solution is required to neutralize the acid present in the solution that helps to increase the pH. Every step on the x-axis consisting of one volume of base. Therefore, the pH change from 4 to 6 on the x-axis is required two-volume of the base.
Want to see more full solutions like this?
Chapter 2 Solutions
BIOLOGY 12E CONNECT ACCESS CARD
- Avidin exists as a protein complex of around 68 kDa. Research to determine the types of interactions that hold the avidin complex together. Based on your research, which molecular weight would you expect for avidin when treated with beta-mercaptoethanol and run through a native gel? 35 kDa 68 kDa 10 kDa 19 kDaarrow_forwardDNA Protein Synthesis Test STRUCTURE OF DNA If 37% of a cell's nucleotides are cytosine, which of the following would also be true? *You may use the calculator function on the sidebar to help with the math. O 13% are adenine O 26% are thymine O 74% are guanine O 74% are adenine 2arrow_forwardThe melting curve for the polyribonucleotide poly(A) is shown below. (a) Explain why absorbance increases with increasing temperature. (b) Why does the shape of the curve differ from the one shown in Fig. ?arrow_forward
- Can you please show me how to work this out and which fraction in the picture provided is correct? Thank you!!arrow_forwardOn an SDS-gel, If the distance traveled by the bromophenol blue dye is 7 cm, and the distance traveled by the protein band is 2.1 cm, the mobility of the protein is 0.3 30 3 30%arrow_forwardch2arrow_forward
- Draw the spectrum of pure DNA at the concentration of 50 ug/ml on the graph provided. List all chromophores present in protein molecules.arrow_forward. Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pk, = 6.0) when the other has a valine (uncharged side chain). (a) Which would be better for separation: gel electrophoresis or isoelectric focusing? Why? (b) What pH would you choose for the separation?arrow_forwardChoices are: Fraction A Fraction B Fraction C Fraction D None of the above All of the above I selected A and was incorrect, I am now lost on how to go about this. Thank You!arrow_forward
- a Mixtureof the following amino acids is subjected to paper electrophoresis: Gly, Arg and Asp.Which of these amino acids will at (a) pH 2.0 and (b) pH 10 move to the Anode (+) and which will move to the cathode(-)? Motivate your answer.arrow_forwardCompute the pI value of tyrosine. If the gel in an electrophoresis experiment is at pH 7, in which direction will tyrosine migrate—toward the cathode or toward the anode? Explain.arrow_forwardTitration curve of an unknown amino acid The graph below shows a curve, which was obtained following titration of an unknown amino acid. Include a suitable descriptive title stating the identity of the unknown amino acid Use rectangles to precisely outline the regions in the graph where ionisable groups show buffering activity (base/acid ratio 1:10 to base/acid ratio 10:1); clearly associate the name of the ionisable group with the buffering regions; indicate within the graph all observable titration mid-points and all observable titration end-points and indicate estimates of the pKa of the three functional groups (do not provide pka values from the literature, you need to read the pka from the titration curve provided). Note that it is not possible to estimate the pka with more than one decimal precision due to limited resolution of the shown graph.arrow_forward
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education