(a) Interpretation: Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK a = 6.0) when the other has a valine (uncharged side chain). The better process for separation: electrophoresis or isoelectric focusing with the required reasons. Introduction: Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA. Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

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Answer 1

Question

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

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Answer 2

Question

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

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Answer 3

Question

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

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Answer 4

Question

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

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Answer 5

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 6

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 7

Chapter 2, Problem 21P

Interpretation Introduction

(a) Interpretation:

Suppose you have two genetic variants of a large protein that differ only in that one contains a histidine (side chain pK_a = 6.0) when the other has a valine (uncharged side chain).

The better process for separation: electrophoresis or isoelectric focusing with the required reasons.

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 8

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 9

Interpretation Introduction

(b) Interpretation:

The pH that would be chosen for the separation

Introduction:

Electrophoresis is a method to separate the macromolecules based on their sizes under the influence of electric field. Gel electrophoresis is used to separate the RNA and DNA.

Isoelectric focusing is a technique to separate the proteins on the basis of pI (at which pH is zero). This is used to separate two proteins of similar molecular weight having different pI.

Answer 10

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Answer 11

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Answer 12

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Answer 13

Ch. 2 - Suppose a chloride ion and a sodium ion are...Ch. 2 - Draw two different possible hydrogen-bonding...Ch. 2 - Prob. 3P Ch. 2 - 4. What is the pH of each of the following...Ch. 2 - Prob. 5P Ch. 2 - The weak acid HA is 2% ionized (dissociated) in a...Ch. 2 - 7. Calculate the pH values and draw the titration...Ch. 2 - What is the pH of the following buffer mixtures?...Ch. 2 - a. Suppose you wanted to make a buffer of exactly...Ch. 2 - Prob. 10P

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