Biochemistry
Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 20, Problem 18P
Interpretation Introduction

To propose:

The unique requirement of FAD in the Succinnate Dehydrogenase.

Introduction:

It is an enzyme complex found in inner membrane of eukaryotes and many bacterial cells.

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Although molecular oxygen (O2) does not participate directly in any of the reactions of the citric acid cycle, the cycle operates only when O2 is present. Explain this observation.
Introduction: As one of the reactants in the first reaction of the citric acid cycle, oxaloacetate is an important cellular metabolite. The concentrations of oxaloacetate are tightly regulated. Different organisms employ different mechanisms to obtain oxaloacetate. In mammals and yeast, oxaloacetate is the product of the pyruvate carboxylase (PYC) reaction. In E. coli, the enzyme phophoenolpyruvate carboxylase (PPC) provides oxaloacetate from phosphoenolpyruvate (PEP) obtained from glucose oxidation in the glycolytic pathway. If glucose is absent and E. coli is using acetate as a carbon source, the glyoxylate pathway serves to generate the needed oxaloacetate. Usually, an organism will employ PPC or PYC, but not both. Detectable levels of PYC in the methanogenic bacterium Methanobacterium thermautotrophicum had previously not been found, and since PPC had been detected, it was believed that M. thermautotrophicum did not possess PYC. However, in the case described here, microbiologists…
Structure, function, Oxidative decarboxylation of pyruvate reactions and mechanisms of activity regulation of pyruvatedehydrogenase multi-enzyme complex.
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