Microbiology: An Introduction
Microbiology: An Introduction
12th Edition
ISBN: 9780321929150
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case
Publisher: PEARSON
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Chapter 20, Problem 6A

The following results were obtained from a broth dilution test for microbial susceptibility.

Antibiotic Concentration Growth Growth in Subculture
200 µg/ml  -  -
100 µg/ml  - +
50 µg/ml + +
25 µg/ml + +

a. The MIC of this antibiotic is _______.

b. The MBC of this antibiotic is _______.

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1. a. Disk A b. Disk B   c. Disk C   d. Disk D   2.  a. Strip A with MIC at 0.25 μg/mL b. Strip B with MIC at 0.064 μg/mL c. Strip C with MIC at 0.075 μg/mL d. All of the strips show equal antimicrobial efficacy
Savlon Extra” disinfectant is known to exhibit good antimicrobial activity against Shigella dysenteriae. At 20oC a concentration of 5% v/v kills 2.0 x106 Sh. dysenteriae cells in 15 minutes. At 25oC the organisms were killed in 5 minutes. a)  Calculate the temperature coefficient of Savlon Extra b). How long will it take the same concentration to kill the same number of Sh. dysenteriae cells at 30oC?
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