Concept explainers
To explain: The role of complementary base-pairing in RT-PCR, DNA microarray analysis, RNA sequencing, and CRISPR-Cas9 editing.
Concept introduction:
The gene functions can be studied through its expression in a particular cell and its interaction with other genes. To study the gene expression, specific mRNAs corresponding to the gene of interest are to be identified. The methods used for these include RT-PCR, DNA microarray analysis, and RNA sequencing. These are based on the base-pairing between the
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