Microorganisms generating methane as a
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Chapter 20 Solutions
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- pls explain Increasing the saturation of the ammonium sulfate is a prerequisite in isolating a target protein that is rich in Cys and Tyr residues. Which of the following techniques should be considered in accurately quantifying the isolated protein?I. Running the isolated protein in a dialysis or GFC set up.II. Using Biuret or BCA assay as the colorimetric quantitation method.III. Using Bradford or Lowry assay as the colorimetric quantitation method.A. I onlyB. II onlyC. I and IIID. I, II and III. Bradford Assay is most suitable to use when the extraction buffer is below the target protein’s pI. This is so because the protein would be morea. Positively charged allowing the CBB G-250 dye to bind via its sulfonate groups.b. Negatively charged allowing the CBB G-250 dye to bind via its sulfonate groups.c. Neutrally charged allowing the CBB G-250 dye to bind via its sulfonate groups.d. Zwitterionic allowing the CBB G-250 dye to bind via its sulfonate groups.arrow_forwardWhat is the expected yield for plasma membranes isolated by sucrose gradient centrifugation from mouse tissues?arrow_forwardneed help to calculate P1 for amino acid Tryptophan. Also please check if I draw the mechanism correctly and completely???arrow_forward
- Quantitatively describe the unique focusing capability of IEF and identify factors that increase resolution of proteins in IEF.arrow_forwardQ1: What is the significance of a large positive ΔG between a substrate and its ES complex and a small ΔG? Q2: What is the significance of a large negative ΔG between a substrate and its Products compared to a small ΔG?arrow_forwardDescribe the molecular mechanism by which quorumsensing controls bioluminescence in V. fischeri.arrow_forward
- Describe the bioreactor design consideration to be taken in account during the bioprocessing of animal cell culture. Please discuss which factors influence the choice of bioreactor design?arrow_forwardhow are SDS-PAGE and BN-PAGE techniques used determine the composition of protein-photosynthetic complexes? also how do they work? please be thorough in the explanation!arrow_forwardSolve: EER= 354- [6.91*24]+ 1.27 * [(9.36*41.8)+(726*1.5748)] = ? Do calculations in the paranthesis first then the bracketsarrow_forward
- We considered the effects of many parameters in designing bioreactors. Please answer the following questions regarding bubbles in airlift fermenters. Bulleted lists are okay. (a) Why was it undesirable to use a very large bubble size (Diameter >7 mm)? (b) Why was it undesirable to use a very small bubble size (Diameter <7 mm)? (c) Other than varying bubble size, what can be done to further decrease cell death due to entrainment?arrow_forwardThe purpose of the progress curve is to determine if an enzymatic reaction rate remains constant for given concentrations of enzyme and substrate, for a given assay time (20 min). How long did the rate of reaction of WGAP remain constant in your experiments? How do you know? What would cause the reaction rate to not be constant and plateau after some time?arrow_forward11) Using the data above, construct Lineweaver-Burk plots. First, graph the inverse of the reaction velocity (rate of reaction) data in column II versus the inverse of the methanol concentration in column I. Then, on the same graph, plot the inverse of the reaction velocity (rate of reaction) data in column III versus the inverse of the methanol concentration in column Iarrow_forward
- BiochemistryBiochemistryISBN:9781305961135Author:Mary K. Campbell, Shawn O. Farrell, Owen M. McDougalPublisher:Cengage Learning
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