Concept explainers
Two patients—a woman and her husband, ages 23 and 22, respectively—arrive at the health clinic one morning. They report having had severe abdominal cramps, grossly bloody diarrhea, nausea, and fever for 48 hours. Cultures of stool samples grown under microaerophilic, capneic conditions contain comma-shaped, Gram-negative bacilli. Both the patients are lactovegetarians and report being part of a “cow leasing” program at a local dairy in which patrons lease part of a cow’s milk production so that they can drink natural, whole, raw milk. The couple devised the program so that they and several neighbors could circumvent state regulations prohibiting the sale of unpasteurized milk. Investigators obtained and cultured a milk sample from the dairy’s bulk milk tank: the cultures contained the bacterium pictured.
- 1. What is the pathogen?
- 2. How did the couple become infected?
- 3. Are the couple's colleagues at work at risk of acquiring an infection from the couple?
- 4. What other foods that are common sources of this bacterium can be ruled out in this case?
Want to see the full answer?
Check out a sample textbook solutionChapter 21 Solutions
Microbiology With Diseases.. -Masteringmic.
- I am having trouble understanding and explaining how I should explain this in lamen terms and which sources can be used to help. Report on : Enterobacter aerogenes Part II – Describe the purpose of identifying unknown bacterial cultures in medical field. Name the step by step experiments/procedures that microbiologists follow in order to identify microorganisms. Part III - MethodsIn this section you are describing in detail all the tests that is done to identify the unknown organism. Gram Stain: Explain differences between gram-positive and Gram-negative organisms. Why One group will end up being purple and another group will be a different color. Very briefly describe the steps of Gram stain. Describe the appearance of your organism under the microscope (shape, color). Tests to identify Gram-negative organisms: List the name of organisms that you are considering. Describe all the tests in the logical and orderly manner. Start by writing the chemical equations; (ALL OF THE CHEMICAL…arrow_forwardThe students of a Microbiology class were tasked to transfer or subculture a pure culture of Escherichia coli bacterium in five 7 mL nutrient broth and five petri dishes of nutrient agar with 20 mL capacity each. Based on the instruction bottles for nutrient broth and nutrient agar, preparation of the culture media is as follows. Nutrient broth: 8 g/liter Nutrient agar: 28 g/liter Answer the following: a. What is the weight in grams of nutrient broth? b. What is the weight in grams of nutrient agar? c. What is the distilled water in mL for nutrient broth? d. What is the distilled water in mL for nutrient agar?arrow_forwardAn outbreak of Methicillin-resistant Staphylococcus aureus (MRSA) in the surgical ward at the hospital forces the hospital administrator to cancel and postpone all non-essential surgeries until the source of infection has been located and for measure put into place. Bacterial samples obtained from swab of wad staff equipment and surfaces arr brought to the lab for analysis. 1. What testing method will you employ to determine the level of exposure? 2 How would a positive test present? 3. How would a negative test present? 4. What recommendations would you make in your report?arrow_forward
- Plastic pipet tips for micropipettes are commonly supplied in packs of 100’s. Once the pack has been opened, contamination is assumed. How can sterility of the pipet tips be ensured prior to use in microbiological procedures? METHOD:RATIONALE:arrow_forwardA student inoculates Escherichia coli into two test tubes containing the same sterile liquid medium, except the first tube also contains a drop of a chemical disinfectant with an antimicrobial effect. After 24 hours of incubation, the first tube remains clear while the second tube becomes cloudy with bacteria. How would you go about determining if the chemical is bacteriostatic or bactericidal?arrow_forwardWhich of the following tests can be used to distinguish between group D streptococcus and other streptococcus bacteria? Mannitol Salt agar Coagulase test McConkey agar Bile eschulin agar Simmon's Citrate agararrow_forward
- A stool specimen of a patient with severe diarrhea was cultured ina series of specialized media for isolation of enteric organisms. Thecultures yielded three isolates that were species of Salmonella, Shigella,and Escherichia. Explain why the H2S production test would be diagnostically significant.arrow_forwardYou want to conduct an microbiological test for a non - aqueous preparation intended for oral use. What is the acceptance criteria for pathogenic microorganism for such preparation? Absence of E Coil Absence of pseudomonas aeruginosa Absence of staphylococcus Aureus 102 ad 101 CFU/G of E Coilarrow_forwardWhat appropriate method should be used in controlling microbial growth in each of the following? 1. Chicken serum 2. Fresh goat milk 3. Nutrient broth 4. Paracetamol 5. Inoculation loobarrow_forward
- Which of the following tests can be used to distinguish between Enterobacter and Pseudomonas strains? blood agar EMB oxidase catalase ureasearrow_forwarddiscuss the clinical significance, principles, reagents (if any) and positive and negative results of the following laboratory tests for Gram positive cocci organisms: Bile esculin test 5% salt turbidity or tolerance test PYRase test Pyruvate broth testarrow_forwardCan you help me create a dichotomous key for these miccoorganisms and tests? Micoorganisms: Bacillus cereus Bacillus subtilis Citrobacter freundii Enterobacter aerogenes Enterococcus faecalis Escherichia coli Klebsiella pneumoniae Micrococcus luteus Mycobacterium phlei Proteus mirabilis Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Staphylococcus aureus Staphylococcus epidermidis Tests: Gram stain, acid-fast stain, endospore stain, amylase, urease, catalase, oxidase,carbohydrates (lactose, sucrose, glucose, mannitol, maltose), MR-VP, SIM, nitrate, motility,citrate, blood agar.arrow_forward
- Microbiology for Surgical Technologists (MindTap ...BiologyISBN:9781111306663Author:Margaret Rodriguez, Paul PricePublisher:Cengage Learning