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Concept explainers
Tay-Sachs Screening. In a certain community, a mutant allele for Tay-Sachs disease (the gene encoding the enzyme hexosaminidase A) exists at a high frequency. This mutation removes a HindIII restriction site (H) in the gene. A probe is available for this region, as shown in Figure 21-33a. Two couples in this community are expecting children. Each couple has a prenatal DNA test to determine if their child will have Tay-Sachs. The results of a Southern blot using HindII digested DNA probed for the region are shown in Figure 21-33b. What advice would you give to each couple in terms of preparing for a child with Tay-Sachs?
(a) Gene structure and probe location
(b) Southern blot
Figure 21-33 Using Southern Blots to Analyze Patients for Tay-Sachs. See Problem 21-4.
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Chapter 21 Solutions
Becker's World of the Cell (9th Edition)
- AAAGAGAAAAGAAUA to AAAGAGAAAUGAAUA. Suppose the codon sequence has a single base pair mutation If the old protein sequence was Lys-Glu-Lys-Arg-Ile, what will be the new sequence encoded by the mutant gene? (Use the 3-letter amino acid abbreviations with hyphens and no spaces in between, i.e. Ser-Asn-Tyr-Leu-Pro.) Submit Answer Retry Entire Group No more group attempts remainarrow_forwardTrue or False. Explain. A) At no time during protein synthesis does an amino acid make direct contact with the mRNA being translated. B) Because the two strands of DNA are complementary, the mRNA of a gene can be synthesized using either strand as a template.arrow_forwardI am more confused. how about we start from begining, you post answers on here, and then we go from there? 1. Identify the open reading frame in the following DNA sequence, the protein that this gene encodes for, its function, and the source. 2. "Look carefully at the DNA sequence and identify the start site for transcription" 3. Click on the DNA sequence from the start site of transcription, select all of the sequence, and copy the sequence. Go to the National Center for Biotechnology Information (NCBI) website http://www.ncbi.nlm.nih.gov/. Click on BLAST on the right-hand side under “Popular Resources.” BLAST is a program that will allow you to find the protein sequence for the DNA sequence (gene) you submit. Next click on blastx (translated nucleotide protein). Paste the DNA sequence into the box under “Entry Query Sequence.” Scroll down and click BLAST. The search may take a few seconds; the page will keep updating until the search is completed. You do not need to enter any…arrow_forward
- Macmillan Learning across generations. Place the events in the order necessary for an epigenetic modification to be inherited in the next generation. $ 4 900 F4 Certain CpG methylation sites are not erased during gametogenesis or embryogenesis. DNA methyltransferase recognizes and binds CpG sites on DNA. A methyl group is added to the cytosine residue of the DNA sequence. DNA methyltransferase maintains the methylation pattern on both DNA strands. % Recent studies have found instances of transgenerational inheritance of epigenetic traits in humans (Relton et al., 2012 already possess their eggs at birth whereas males do not produce sperm until nuberty For enigenetic modifications to 5 F5 ^ Methylated CpG sites on one inherited DNA strand 6 Epigenetic silencing passed to offspring F6 MacBook Air Answer Bank & 7 F7 * 8 DII. F8 DD F9 F10 J F11 Uarrow_forwardTrue or False? Eukaryotic genomes are organized into operons; each operon consists of a series of genes which code for enzymes involved in a metabolic pathway, under the transcriptional control of a single promoter sequence .arrow_forwardtransformation and CRISPR. In your own words, briefly describe two differences between these technologies. (For example, these can be differences between their outcomes, procedures, reagents, or something else.)arrow_forward
- draw the p21 promoter. Your drawing should include (1) the start site, (2) the TATA box and (3) the ERE/AP-1 binding sitearrow_forwardto only 23 rounds for a woman of age 20. That is a 6.5-fold greater number of cell divisions and proportionately greater opportunity for new point mutations. Yet, on average, 20-year-old men contribute only about twice as many new point mutations to their offspring as do women. How can you explain this discrepancy?arrow_forwardResearch cancer mutation. Provide the link to the research article that gives you your information. One good resource to use is PubMed. Then answer the following questions, in 3 paragraphs, 3-5 sentences each. 1. What kind of disease/cancer does this mutation cause? 2.What happens during transcription to cause this mutation? 3. Is this trait passed on to progeny? Can the progeny be a carrier or simply affected?arrow_forward
- GTTTTCACTGGCGAGCGTCATCTTCCTACT 8. What is the function (e.g. transcriptional regulation, transmembrane signaling, kinase, protease, etc.) of the protein(s) encoded by the gene.arrow_forwardMatch the term with its description. cAMP Allows lactose to enter the cell and product of the lacY gene A regulatory sequence between the promoter and the structural genes of the lac operon When glucose levels are high, intracellular levels of this molecule are low Binds to and inhibits the function of the lac repressor when lactose is presentarrow_forwardMATCH THE FF. WITH THE CHOICES BELOW group of genes one long mRNA with complementary codes of 3 or more genes controlled by negative regulation a tetrameric protein, is the lacI gene product It is a palindromic DNA segment at the upstream region of lac gene has a DNA-binding domain on N-terminus; the C-terminus binds inducer, forms tetramer. an accessory protein to activate transcription regulated both positively and negatively by AraC regulatory elements of arabinose operon It is Regulated Through a Co-Repressor-Mediated Negative Control Circuit an example of autogenous regulation has attenuation regulation mechanism CHOICES: -lac operator -Catabolite activator protein -araBAD Operon -polycistronic mRNA -trp Operon -lac operon -lac repressor -Operon -AraC genearrow_forward
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