Chapter 21, Problem 21.55E

Interpretation Introduction

Interpretation:

The way by which heating a double-stranded DNA fragment to $94\text{°C}-96\text{°C}$ when performing PCR causes the DNA to unravel into single strands is to be stated.

Concept introduction:

DNA is a type of nucleic acid and stands for deoxyribonucleic acid. It is found primarily in nuclei of the cells and is present in the form of long chain polymers. It has a double helix structure in which the nucleotides are joined together by phosphate groups that join the $5\text{'}$ carbon of one nucleotide to the $3\text{'}$ carbon of the other nucleotide. PCR technique imitates the natural process of replication of DNA.