Concept explainers
Interpretation:
The elution order of given amino acids with
Concept introduction:
Ion-exchange chromatography is used to separate mixture of amino acids on the basis of their overall charge by using cation or anion-exchange resins.
Dowex-1 is an anion-exchange resins with less polar used for anion-exchange chromatography.
Anion-exchange chromatography separate amino acids according to their
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EBK ORGANIC CHEMISTRY
- 22-49 Based on your knowledge of the chemical properties of amino acid side chains, suggest a substitution for leucine in the primary structure of a protein that would probably not change the character of the protein very much.arrow_forward22-61 Polyglutamic acid (a polypeptide chain made only of glutamic acid residues) has an a-helix conformation below pH 6.0 and a random-coil conformation above pH 6.0. What is the reason for this conformational change?arrow_forward22-71 Which amino acid side chain is most frequently involved in denaturation by reduction?arrow_forward
- 22-6 The members of which class of proteins are insoluble in water and can serve as structural materials?arrow_forward22-45 Proteins are least soluble at their isoelectric points. What would happen to a protein precipitated at its isoelectric point if a few drops of dilute HCl were added?arrow_forwardExplain why a protein is least soluble in an aqueous medium that has a pH equal to the isoelectric point of the protein.arrow_forward
- A direct current was passed through a solution containing valine histidine and aspartic acid at a pH of 6.0 .One amino acid migrated to the poritive electrode ,one migrated to negative electrode and one did not migrate to either electrode .Which amino acids went where?arrow_forwardWrite down the structure of glutamic acid in each pH range from acidic to basic. It is known that pKa1 = 2.5 pKa2 = 3.5 pKa3 = 8.7 and draw the titration curve of glutamic acid titrated with a strong base, such as NaOH.arrow_forwardFor aspartic and glutamic acids, the isoelectric point occurs at a pH where the net charge on the two carboxyl groups is -1 and balances the charge of +1 on the a-amino group. Calculate pI for these amino acids.arrow_forward
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