(a)
Interpretation:
Requirement of high pressure in HPLC has to be explained.
Concept Introduction:
High performance liquid chromatography in
(b)
Interpretation:
Reason behind the increase in efficiency of liquid chromatography with decrease in particle size of the stationary phase has to be explained.
Concept Introduction:
Plate height can be calculated by van Deemter equation as follows:
Here
The term
The term
The term
Plate height is inversely proportional to the efficiency of the chromatographic separation.
(b)
Interpretation:
Advantages and disadvantages of UPLC have to be explained.
Concept Introduction:
Ultra performance liquid chromatography is a modification over HPLC. It is applicable for particles with less than
(d)
Interpretation:
Advantages of the use of superficially porous particle has to be written.
Concept Introduction:
In superficially porous particle chromatography, stationary phase diffuses into fully porous particles with a radius of
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