Concept explainers
(a)
Interpretation:
The effect of enzyme mutation on enzyme activity with varying concentrations of carnitine must be determined.
The KM and Vmax values of wild-type and mutant enzymes must be determined.
Concept introduction:
Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.
(b)
Interpretation:
The effect of enzyme mutation on enzyme activity with varying concentrations of palmitoyl CoA must be determined.
The KM and Vmax values of wild-type and mutant enzymes must be determined.
Concept introduction:
Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.
(c)
Interpretation:
The enzyme that is more sensitive to malonyl CoA inhibition must be determined.
Concept introductionCarnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.
(d)
Interpretation:
Effect of 100 µM palmitoyl CoA, 100 µM carnitine, and malonyl CoA 10µM on mutant enzyme and wild type enzyme must be determined.
Concept introduction:
Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.
(e)
Interpretation:
Role of glutamate 3 in CPTI function should be determined.
Concept introduction:
Carnitine palmitoyl transferase I is a mitochondrial enzyme, which forms acyl carnitine by catalyzing the reaction between acyl CoA, and l-carnitine. This allows the transfer of fatty acid chain from cytosol to the mitochondria of the cell. there are three isoforms of CPT I enzyme, CPT1A, CPT1B, and CPT1C.
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BIOCHEMISTRY W/1 TERM ACHEIVE ACCESS
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- BiochemistryBiochemistryISBN:9781305577206Author:Reginald H. Garrett, Charles M. GrishamPublisher:Cengage Learning