Concept explainers
Interpretation:
The sticky ends that result from cleavage of the double stranded DNA segment with BamH1 should be drawn.
Concept Introduction:
Restriction endonucleases recognizes a specific
Want to see the full answer?
Check out a sample textbook solutionChapter 22 Solutions
GENERAL ORGANIC & BIOCHEMISTRY >ACCESS<
- The -chain of hemoglobin is a protein that contains 146 amino acids residues. What minimum number of nucleotides must be present in a strand of mRNA that is coded for this protein?arrow_forwardProtein synthesis begins when a ribosome assembles at an initiation codon (AUG) in the mRNA. The completed protein chain is released from the ribosome when an initiation codon (UAA, UAG, or UGA) is reached a. Both of the statements are TRUE b. Both of the statements are FALSE c. Statement I is TRUE, Statement II is FALSE d. Statement I is FALSE, Statement II is TRUEarrow_forwardOne strand of DNA has the base sequence: C G A T T G G C A G T C A T. Determine the sequence of bases in the complementary strand of mRNA that would form next to this DNA strand.arrow_forward
- b) Suppose a mutation in an enzyme resulted in the replacement of a leucine with a serine in the active site of the enzyme (so you have a serine where you should have a leucine). How might that affect substrate binding?arrow_forwardHere is a portion of the nucleotide sequence in phenylalanine tRNA. 3'-ACCACCUGCUCAGGCCUU-5' Write the nucleotide sequence of its DNA complementarrow_forwardRetroviruses like HIV, the pathogen responsible for AIDS, incorporate an RNA template that is copied into DNA duringinfection. The reverse transcriptase enzyme that copies RNA into DNA is relatively nonselective and error-prone, leadingto a high mutation rate. Its lack of selectivity is exploited by the anti-HIV drug AZT (3′@azido@2′,3′@dideoxythymidine),which becomes phosphorylated and is incorporated by reverse transcriptase into DNA, where it acts as a chain terminator.Mammalian DNA polymerases are more selective, having a low affinity for AZT, so its toxicity is relatively low.(a) Draw the structures of AZT and natural deoxythymidine.(b) Draw the structure of AZT 5′@triphosphate, the derivative that inhibits reverse transcriptase.*2arrow_forward
- For the polymerase chain reaction to occur, ____. a. a large sample of DNA is needed b. a sticky end must be available for the ligase enzyme to function c. the DNA must remain double stranded d. the reaction temperature must be cycled from low to high e. all DNA fragments must be identicalarrow_forwardWhich statement BEST describes why the sugar phosphate nucleic acid backbone is on the outer part of the DNA double helix while the nitrogenous bases are on the inside of the double helix? O The sugar phosphate backbone is hydrophilic while the bases are less polar. ○ The sugar phosphate backbone is hydrophobic while the bases are hydrophilic. The nitrogen bases are covalently bonded to each other. O There is no reason; the structure is just that way.arrow_forwardReplication of DNA produces two daughter DNA molecules in which a. once daughter molecules contains both parent strands and one daughter molecule contains both newly synthesized strands b. each daughter molecule contians one parent strand and one newly synthesized strand c. each daughter molecule contians two newly synthesized strands d. each daughter molecule contians both parent strandsarrow_forward
- DNA Replication1) Construct the complementary strand of DNA (strand II) that would pair with the DNA strand I sequence below. Remember that nucleotides are always added in the 5 prime to 3 prime direction. The base pairing rules are: DNA DNAadenine (A) always bonds with thymine (T) thymine (T) always bonds with adenine (A) guanine (G) always bonds with cytosine (C) cytosine (C) always bonds with guanine (G) STRAND I: 3’ T A C T A T A G T C T G T C T C C C A C T 5’ STRAND II: 5’ 3’arrow_forwardDuring DNA replication, which of the following enzymes acts as the "workhorse," inserting DNA nucleotides to create new strands of DNA? O a. Ligase O b. DNA Polymerase III O c. Primase Od. Helicase e. DNA Polymerase Iarrow_forwardIf PCR and DNA replication are very synonymous processes, what components are lacking in PCR requiring it to have varying temperatures whereas DNA replication may occur in normal physiological temperatures?arrow_forward
- General, Organic, and Biological ChemistryChemistryISBN:9781285853918Author:H. Stephen StokerPublisher:Cengage LearningOrganic And Biological ChemistryChemistryISBN:9781305081079Author:STOKER, H. Stephen (howard Stephen)Publisher:Cengage Learning,Chemistry & Chemical ReactivityChemistryISBN:9781337399074Author:John C. Kotz, Paul M. Treichel, John Townsend, David TreichelPublisher:Cengage Learning
- Chemistry for Today: General, Organic, and Bioche...ChemistryISBN:9781305960060Author:Spencer L. Seager, Michael R. Slabaugh, Maren S. HansenPublisher:Cengage LearningIntroductory Chemistry: An Active Learning Approa...ChemistryISBN:9781305079250Author:Mark S. Cracolice, Ed PetersPublisher:Cengage Learning