Microbiology: An Evolving Science (Fourth Edition)
4th Edition
ISBN: 9780393615098
Author: John W. Foster, Joan L. Slonczewski
Publisher: W. W. Norton & Company
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Chapter 2.4, Problem 1TQ
Summary Introduction
To review:
The experiment to determine the actual order of events of DNA (Deoxyribonucleic acid) replication in Bacillus subtilis.
Introduction:
Bacterial DNA is also a double-helical DNA but is not found as a thread or in the linear form. It is found to be in circular form. The DNA is comprised of purines and pyrimidine. The bacterial DNA is in different arrangements as compared to the DNA of a eukaryotic cell but the events of
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What are the three models of DNA replication? With the aid of illustrations, show how the Meselson Stahl experiment come to the conclusion of one model of DNA replication.
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Chapter 2 Solutions
Microbiology: An Evolving Science (Fourth Edition)
Ch. 2.1 - Prob. 1TQCh. 2.1 - Prob. 2TQCh. 2.2 - Prob. 1TQCh. 2.3 - Prob. 1TQCh. 2.3 - Prob. 2TQCh. 2.4 - Prob. 1TQCh. 2.5 - Prob. 1TQCh. 2.5 - Prob. 2TQCh. 2.6 - Prob. 1TQCh. 2.6 - Prob. 2TQ
Ch. 2 - Prob. 1RQCh. 2 - Prob. 2RQCh. 2 - Prob. 3RQCh. 2 - Prob. 4RQCh. 2 - Prob. 5RQCh. 2 - Prob. 6RQCh. 2 - Prob. 7RQCh. 2 - Prob. 8RQCh. 2 - Prob. 9RQCh. 2 - Prob. 10RQCh. 2 - Prob. 11RQCh. 2 - Prob. 12RQCh. 2 - Prob. 13RQCh. 2 - Prob. 14RQCh. 2 - Prob. 15RQCh. 2 - Prob. 1TQCh. 2 - Prob. 2TQCh. 2 - Prob. 3TQCh. 2 - Prob. 4TQ
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- What is the purpose and benefit of the polymerase chain reaction?arrow_forwardIn the “How we know” regarding the origin of replication and cell survival on ampicillin, why does the absence of an origin of replication lead to no colonies on the left plate?arrow_forwardWhat are the differences between In vitro and In vivo DNA replication? Please answer at your own words.arrow_forward
- If Meselson and Stahl had first grown the cells in 14N-containingmedium and then moved them into 15N-containing medium before takingsamples, what would have been the result after each of the two replications?arrow_forwardYou conducted an experiment to determine the mechanism of DNA replication in the hypothetical organism Fungus mungus. Your data shows that synthesis of newly replicated DNA from F. mungus is discontinuous on both strands of the replication fork. Does this result support or not support the hypothesis that F. mungus replicates its DNA by the same mechanism as yeast? Briefly explain your answer.arrow_forwardHow many different temperatures are used in each cycle of the polymerase chain reaction?arrow_forward
- Suppose that replication is initiated in a medium containing moderately radioactive tritiated thymine. After a few minutes of incubation, the bacteria are transferred to a medium containing highly radioactive tritiated thymidine. Sketch the autoradiographic pattern that would be seen for (a) undirectional replication and (b) bidirectional replication, each from a single origin.arrow_forwardwhich step of the polymerase chain reaction takes place at 98 degrees?arrow_forwardList and describe the steps in prokaryotic DNA replication. How does this process appear to differ from eukaryotic DNA replication?arrow_forward
- explain the term semiconservative replication?arrow_forwardA bacterium undergoes four rounds of replication. How many cells would result, and how many of those cells would still have part of an original DNA strand from the starting bacterium?arrow_forwardThe above experiment, on DNA synthesis in the intact chromosomes of E. coli (with no virus infection), demonstrates which of the following forms of DNA replication? completely discontinuous replication completely conservative replication completely dispersive replication semi-discontinuous replication semi-conservative replicationarrow_forward
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