a)
Interpretation:
The adjusted retention time and retention factor has to be calculated.
Concept Introduction:
Adjusted retention time:
The extra time that is required to travel the length of the column beyond which is required by the solvent is called as adjusted retention time. The adjusted retention time is calculated using the equation,
Where,
Retention factor:
The time that is necessary to elute that peak minus the minimum possible time for the travel of unretained solute through the column is called as retention factor. The retention factor is calculated using the equation,
Where,
To calculate the adjusted retention time and retention factor
a)
Answer to Problem 24.15P
The adjusted retention time is
The retention factor is
Explanation of Solution
Given,
The adjusted retention time is calculated as,
The adjusted retention time =
The retention factor is calculated as,
The retention factor =
b)
Interpretation:
The phase ratio
Concept Introduction:
Phase ratio
The volume of mobile phase divided by the volume of the stationary phase is called as dimensionless phase ratio. The phase ratio is calculated as,
Where,
Increase in thickness of stationary phase, decreases in
To calculate the phase ratio
b)
Answer to Problem 24.15P
The phase ratio
Explanation of Solution
Given,
The phase ratio is calculated as,
The phase ratio
c)
Interpretation:
The partition coefficient for the analyte has to be calculated.
Concept Introduction:
Partition coefficient:
The partition coefficient for the analyte is calculated using the formula of retention factor,
Here,
The formula for calculating the partition coefficient for the analyte is,
To calculate the partition coefficient for the analyte
c)
Answer to Problem 24.15P
The partition coefficient for the analyte is
Explanation of Solution
Given,
Retention factor =
Phase ratio
The partition coefficient for the analyte is calculated as,
The partition coefficient for the analyte =
d)
Interpretation:
The retention time for the given length and thickness has to be calculated.
Concept Introduction:
Retention factor:
The time that is necessary to elute that peak minus the minimum possible time for the travel of unretained solute through the column is called as retention factor. The retention factor is calculated using the equation,
Where,
The retention time can be calculated using the formula of retention factor,
Here,
Phase ratio:
The volume of mobile phase divided by the volume of the stationary phase is called as dimensionless phase ratio. The phase ratio is calculated as,
Where,
Increase in thickness of stationary phase, decreases in
To calculate the retention time
d)
Answer to Problem 24.15P
The retention time is calculated to be
Explanation of Solution
Given,
The partition coefficient for the analyte =
The phase ratio is calculated as,
The phase ratio
The retention time is calculated as,
The retention time =
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