(a)
Interpretation:
The separation of Blue Dextran has 2000 in gel filtration column using should be explained.
Concept introduction:
Gel filtration in open columns:
In the gel filtration column, the pore size of the stationary phase is chosen depends on the molecular mass of the molecules to be separated.
The fractionation range for globular proteins (Da) are used as gel in gel filtration columns and more size of gel are available based on it is size so the stationary phase size (Da) is depends on molar mass of the compound, which is going to separate.
(b)
Interpretation:
The retention volume of a molecule in gel-filtration column should be determined.
Concept introduction:
The volume of column is given by the mathematical expression it is,
Where,
The Elution Equation:
Where,
Want to see the full answer?
Check out a sample textbook solutionChapter 26 Solutions
Solution Manual for Quantitative Chemical Analysis
- ChemistryChemistryISBN:9781305957404Author:Steven S. Zumdahl, Susan A. Zumdahl, Donald J. DeCostePublisher:Cengage LearningChemistryChemistryISBN:9781259911156Author:Raymond Chang Dr., Jason Overby ProfessorPublisher:McGraw-Hill EducationPrinciples of Instrumental AnalysisChemistryISBN:9781305577213Author:Douglas A. Skoog, F. James Holler, Stanley R. CrouchPublisher:Cengage Learning
- Organic ChemistryChemistryISBN:9780078021558Author:Janice Gorzynski Smith Dr.Publisher:McGraw-Hill EducationChemistry: Principles and ReactionsChemistryISBN:9781305079373Author:William L. Masterton, Cecile N. HurleyPublisher:Cengage LearningElementary Principles of Chemical Processes, Bind...ChemistryISBN:9781118431221Author:Richard M. Felder, Ronald W. Rousseau, Lisa G. BullardPublisher:WILEY