Inquiry Into Life (16th Edition)
16th Edition
ISBN: 9781260231700
Author: Sylvia S. Mader, Michael Windelspecht
Publisher: McGraw Hill Education
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Chapter 26.1, Problem 1AQTC
Summary Introduction
To analyze:
The consequence of unintentional breaks in DNA sequences other than the target sequence due to CRISPR.
Introduction:
CRISPR or Clustered Regularly Interspaced Short Palindromic Repeats are DNA sequences present in prokaryotes. They along with the enzyme Cas9 are part of the defense system of prokaryotes. Now, the CRISPR-Cas9 system is used as a gene-editing tool. It can break DNA strands at the desired location where the gene sequence can be modified according to our requirement.
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What is the function of the CRISPR/Cas system?
What are the ethical impacts of a technology such as CRISPR?
Attached is a cartoon model of the CRISPR system, with 3 critical components labeled. A. What is the identity of each component?
B. What is the function of component a?
C. What is the function of component c?
Why does Northern blotting not require the use of restriction enzymes?
a. Restriction enzymes cut at specific sites within DNA
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Chapter 26 Solutions
Inquiry Into Life (16th Edition)
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1AQTCCh. 26.1 - Prob. 2AQTC
Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 2LOCh. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 3CYPCh. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - The polymerase chain reaction Use RNA polymerase...Ch. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - In this process, cells are removed from the body...Ch. 26 - When a cloned gene is used to modify a human...Ch. 26 - Prob. 8ACh. 26 - Prob. 9ACh. 26 - Prob. 10ACh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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Need a deep-dive on the concept behind this application? Look no further. Learn more about this topic, biology and related others by exploring similar questions and additional content below.Similar questions
- Besides the great potential of gene editing, what are the biggest risks?  How do scientist test to ensure their gene editing experiments target only the desired specific genes?arrow_forwardWhat would happen if the restriction enzymes do not cut the DNA at specific recognition sequences?arrow_forwardDoes one have a practical idea of the role of CRISPR/Cas9 in genome editing via cutting of DNA?arrow_forward
- What would be some major impediments to genetically modifyinghuman embryos with CRISPR?arrow_forwardIn cloning human DNA, why is it necessary to insert the DNA into a vector such as a bacterial plasmid?arrow_forwardWhat advantages do cDNA libraries provide over genomic DNA libraries? Describe cloning applications where the use of a genomic library is necessary to provide information that a cDNA library cannot.arrow_forward
- What are the major components of the CRISPR-Cas9 system? What mechanism does it employ to combine DNA? Explain the process of how the CRISP-Cas9 system is able to create recombinant DNA. Relate the idea of gene modification to the fields of vaccines and applied microbiology as well.arrow_forwardHow many fragments would you expect to be formed from digestion of a 2500 base pair long linear piece of DNA using a restriction enzyme with a 5 base pair recognition sequence?”arrow_forwardIn making recombinant DNA, what is the benefit of using a restriction enzyme that cuts DNA in a staggered fashion?arrow_forward
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