Inquiry Into Life (16th Edition)
16th Edition
ISBN: 9781260231700
Author: Sylvia S. Mader, Michael Windelspecht
Publisher: McGraw Hill Education
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Chapter 26, Problem 3A
The polymerase chain reaction
- Use RNA polymerase
- takes place in huge bioreactors.
- uses a temperature- insensitive enzyme
- Makes lots of nonidentical copies of DNA
- All of these are correct
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What special property does Taq polymerase have?
It binds to each strand of DNA after the two strands of DNA have separated.
It is highly specific for bacterial DNA.
It can tolerate a higher temperature.
It allows the DNA double helix to open.
It requires less ATP to function than other polymerases.
DNA polymerases ___.
catalyze carbon bonding
seal gaps in the sugar-phosphate backbone
add new nucleotides to a strand
repair DNA
Polymerase chain reaction mimics the cells ability to:
translate mRNA into protein
transcribe dna to mrna
replicates dna
repair dna
edit dna
Chapter 26 Solutions
Inquiry Into Life (16th Edition)
Ch. 26.1 - Describe the steps in forming Recombinant DNA.Ch. 26.1 - Discuss how the polymerase chain reaction works.Ch. 26.1 - Prob. 3LOCh. 26.1 - Prob. 1QTCCh. 26.1 - Prob. 2QTCCh. 26.1 - Prob. 1CYPCh. 26.1 - Explain how the PCR reaction amplifies a segment...Ch. 26.1 - Prob. 3CYPCh. 26.1 - Prob. 1AQTCCh. 26.1 - Prob. 2AQTC
Ch. 26.2 - Prob. 1LOCh. 26.2 - Prob. 2LOCh. 26.2 - Prob. 1QTCCh. 26.2 - Prob. 2QTCCh. 26.2 - Prob. 3QTCCh. 26.2 - Prob. 1CYPCh. 26.2 - Prob. 2CYPCh. 26.3 - Compare and contrast in vivo and ex vivo gene...Ch. 26.3 - Prob. 2LOCh. 26.3 - Prob. 1CYPCh. 26.3 - Prob. 2CYPCh. 26.3 - Prob. 3CYPCh. 26.4 - Prob. 1LOCh. 26.4 - Prob. 2LOCh. 26.4 - Prob. 3LOCh. 26.4 - Prob. 1CYPCh. 26.4 - Explain how comparative genomics can provide...Ch. 26.4 - Prob. 3CYPCh. 26 - Prob. S25.1BYBCh. 26 - Prob. S25.2BYBCh. 26 - Prob. S25.3BYBCh. 26 - Prob. 1ACh. 26 - Prob. 2ACh. 26 - The polymerase chain reaction Use RNA polymerase...Ch. 26 - Prob. 4ACh. 26 - Prob. 5ACh. 26 - In this process, cells are removed from the body...Ch. 26 - When a cloned gene is used to modify a human...Ch. 26 - Prob. 8ACh. 26 - Prob. 9ACh. 26 - Prob. 10ACh. 26 - Prob. 1TCCh. 26 - In a genomic comparison between humans and yeast,...
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- What is the function of DNA polymerase? a. It degrades DNA in cells. b. It adds RNA nucleotides to a new strand. c. It coils DNA around histones to form chromosomes. d. It adds DNA nucleotides to a replicating strand. e. None of these.arrow_forwardCloning Genes Is a Multistep Process Which enzyme is responsible for covalently linking DNA strands together? a. DNA polymerase b. DNA ligase c. EcoRl d. restriction enzymes e. RNA polymerasearrow_forwardDNA replicates by which of the following models? a. conservative b. semiconservative c. dispersive d. none of the abovearrow_forward
- Energy that drives the attachment of a nucleotide to the end of a growing strand of DNA comes from ________. a. the nucleotide b. DNA polymerase c. phosphate-group transfers from ATParrow_forwardFigure 4.10 Enzymes, temperature, and pH. Each enzyme works best within a characteristic range of conditionsgenerally the same environmental conditions in which the enzyme normally occurs. Figure It Out: At what temperature does the E.coli DNA polymerase work fastest?arrow_forwardDNA replication requires ________ . a. DNA polymerase c. primers b. nucleotides d. all are requiredarrow_forward
- In a PCR reaction, a few seconds at high temperature disrupts the ____ that hold the two strands of DNA together, so every molecule of DNA unwinds and becomes single-stranded. DNA ligases covalent bonds peptide bonds hydrogen bonds lipoproteinsarrow_forwardWhy is the following statement fals? RNA polymerase is a dangerous enzyme to the cell because it does not proofread as well as DNA polymerasearrow_forwardThe best explanation for why DNA synthesis is discontinuous would be that ____. DNA polymerase can only move along the DNA strand in one direction This allows for efficient error checking of newly synthesized DNA DNA polymerase must stop periodically to reload more nucleotides None of the abovearrow_forward
- The synthesis of new DNA requires energy. Where does this needed energy come from? The DNA polymerase reading the old DNA strand. The triphosphate nucleotide that is being added to the new DNA strand. The carbohydrates that the bacteria cell eats. The breaking of the hydrogen bonds between the opposite DNA strands.arrow_forwardDNA can be unwound from the double stranded double helix into single strands, amplified, separated on gels by size, stained with dyes. It can be mutated by a variety of means. It can be sequenced. Describe one or more of the ways that scientists are able to manipulate DNA and relate this to the structure of the DNA.arrow_forwardFill in the blanks in the paragraph about DNA replication. There are many _____________ involved in DNA replication. _____________ unwinds the DNA strand while _____________ breaks the hydrogen bonds between bases. _____________ lays down an RNA primer that lets ________________ know where to begin adding new nucleotides. The _______________ is made continously, while the _________________ is made in fragments called __________________. These fragments are glued together by ______________.arrow_forward
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