BIO 1406/07 W/CONNECT/LM NEW >IC<
16th Edition
ISBN: 9781260075762
Author: Raven
Publisher: MCG CUSTOM
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Textbook Question
Chapter 27, Problem 5S
How might phage λ be used to transfer E. coli genes between different bacterial cells? Could this be used to transfer any gene?
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What is horizontal gene transfer? What are the three mechanisms for this to occur in bacteria?
What are the components needed for the processes of transformation, conjugation, and transduction? How does each process occur? What genes are involved in each process?
How do generalized and specialized transduction differ? What is the end result of each?
What is recombination? What is the importance to bacteria & archaea?
What are the two types of recombination? What are the details of each type? What components are needed for each type?
What is a transducing phage?
The figure above shows a schematic of genes and transcription control elements from phage λ. Use this figure as an aid to help you describe the molecular events involved in:
a) The establishment of lysogeny
b) The establishment of a lytic life cycle
Chapter 27 Solutions
BIO 1406/07 W/CONNECT/LM NEW >IC<
Ch. 27 - The reverse transcriptase enzyme is active in...Ch. 27 - Which of the following is NOT part of a virus? a....Ch. 27 - Which of the following is common in animal viruses...Ch. 27 - Which of the following would NOT be part of the...Ch. 27 - A process by which a virus may change a benign...Ch. 27 - Prob. 6UCh. 27 - Prob. 7UCh. 27 - Prob. 1ACh. 27 - The idea of a protein that was an infectious agent...Ch. 27 - Bacterial viruses and animal viruses are similar...
Ch. 27 - Prob. 4ACh. 27 - Phage conversion in which viruses add genes to a...Ch. 27 - According to the prion hypothesis, the infectious...Ch. 27 - Prob. 7ACh. 27 - Prob. 8ACh. 27 - E. coli lysogens derived from infection by phage ...Ch. 27 - Most biologists believe that viruses evolved...Ch. 27 - Prob. 3SCh. 27 - What do we mean by the term emerging virus? How is...Ch. 27 - How might phage be used to transfer E. coli genes...
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- After a phage injects its DNA into a bacterial cell, the cell begins making proteins that make up the phage coat. Why does the same thing not happen when a generalized transducing particle injects the DNA it carries?arrow_forwardWhy is phage conversion considered beneficial to host cells?arrow_forwardWhat is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance.arrow_forward
- Is a phage a bacteria?arrow_forwardIf we were to examine a strain with the F plasmid inserted into the same site of the bacterial chromosome, but in the reverse orientation: a) What would the order of gene transfer be? Include all of the genetic markers including the amino acid and nucleic acid metabolism genes and streptomycin resistance. b) What cell types would be able to grow on the NA vs ECM media types? Be sure to include the genotypes of the cells that would grow. Remember that NA provides all nutrients the bacteria needs + no antibiotic and HCM = minimal medium + glucose + has streptomycin antibiotic c) Would we still be able to perform our mapping? Why or why not? (Hint: refer to part b above)arrow_forwardOne of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?arrow_forward
- When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?arrow_forwardConsider three genes in E. coli: thr+, ara+, and leu+ (which give the cell the ability to synthesize threonine, arabinose, and leucine, respectively). All three of these genes are close together on the E. coli chromosome. Phages are grown in a thr+ ara+ leu+ strain of bacteria (the donor strain). The phage lysate is collected and used to infect a strain of bacteria that is thr− ara− leu −. The recipient bacteria are then tested on selective medium lacking leucine. Bacteria that grow and form colonies on this medium (leu+ transductants) are then replica-plated on medium lacking threonine and on medium lacking arabinose to see which are thr+ and which are ara+. Another group of the recipient bacteria are tested on medium lackingthreonine. Bacteria that grow and form colonies on this medium (thr+ transductants) are then replica-plated on medium lacking leucine and onto medium lacking arabinose to see which are ara+ and which are leu+. Results from these experiments are as follows:…arrow_forwardIn a P1 transduction experiment, the P1 lysate contains phages thatcarry pieces of the host chromosomal DNA, but the lysate also containsbroken pieces of chromosomal DNA. If a P1 lysate is used to transfer chromosomal DNA to another bacterium, how could you show experimentally that the recombinant bacterium has been transduced (i.e., has taken up a P1 phage with a piece of chromosomal DNA inside) versus transformed (i.e., has taken up a piece of chromosomal DNA that is not within a P1 phage coat)?arrow_forward
- Lambda phage çan facilitate bacterial chemosomal DNA transfer through the process of specialized transduction / generalized transduction (copy and paste the correct answer) A by transferring specific bacterial DNA / bacterial DNA at random (copy and paste the correct answer) A from a donor cell / recipient cell (copy and paste the correct answer) A to a donor cell / recipient cell (copy and paste the correct answer).arrow_forwardWhat is pGLO plasmid? How has it been engineered so that the cells that carry it can be detected? Attach a map of it.?arrow_forwardWhen would it be beneficial to use a eukaryotic host formolecular cloning?arrow_forward
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