EBK BROCK BIOLOGY OF MICROORGANISMS
15th Edition
ISBN: 8220103633352
Author: Stahl
Publisher: PEARSON
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Textbook Question
Chapter 28.6, Problem 2MQ
- What are the advantages and disadvantages of agglutination tests versus fluorescent antibody assays? How are the latter used to identify specific cells in complex mixtures, such as blood?
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What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?
ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or falsenegative reactions)?
What are the two types of ELISA methods and how do they work? What is a chromogen?
Chapter 28 Solutions
EBK BROCK BIOLOGY OF MICROORGANISMS
Ch. 28.1 - The use of personal protective equipment (PPE) is...Ch. 28.1 - Identify and discuss the standard safety...Ch. 28.1 - Prob. 1CRCh. 28.2 - Prob. 1MQCh. 28.2 - How can the spread of HAIs be controlled?Ch. 28.2 - Prob. 1CRCh. 28.3 - What are the key points necessary for proper...Ch. 28.3 - Identify culture methods and conditions used for...Ch. 28.3 - QWhy is it important to process clinical specimens...Ch. 28.4 - Describe the disc diffusion test and the Etest for...
Ch. 28.4 - What is the value of antimicrobial drug...Ch. 28.4 - QDescribe the disc diffusion test for antibiotic...Ch. 28.5 - Explain the reasons for changes in antibody titer...Ch. 28.5 - Describe the method, time frame, and rationale for...Ch. 28.5 - What advantages do monoclonal antibodies have...Ch. 28.5 - QWhy does antibody titer rise after infection? Is...Ch. 28.6 - How is the bivalence of antibodies significant for...Ch. 28.6 - What are the advantages and disadvantages of...Ch. 28.6 - Why are agglutination tests so widely used in...Ch. 28.7 - Prob. 1MQCh. 28.7 - Compare the advantages and disadvantages of EIA,...Ch. 28.7 - Prob. 1CRCh. 28.8 - What advantage(s) does nucleic acid amplification...Ch. 28.8 - How do quantitative PCR (qPCR) and qualitative PCR...Ch. 28.8 - Distinguish between quantitative and qualitative...Ch. 28.9 - Compare and contrast live attenuated vaccines,...Ch. 28.9 - Identify the advantages of alternative...Ch. 28.9 - QList the immunizations recommended for children...Ch. 28.10 - Prob. 1MQCh. 28.10 - How does the activity of each antibiotic class...Ch. 28.10 - What are the sources of aminoglycosides,...Ch. 28.10 - Antibiotics are chemically diverse antimicrobial...Ch. 28.11 - What steps in the viral maturation process are...Ch. 28.11 - Why are there fewer clinically effective...Ch. 28.11 - Why is host toxicity a common problem with...Ch. 28.12 - Identify the basic mechanisms of antibiotic...Ch. 28.12 - What does vancomycin have in common with...Ch. 28.12 - Prob. 3MQCh. 28.12 - What practices contribute to the spread of...Ch. 28 - Define the procedures you would use to isolate and...Ch. 28 - Prob. 2AQCh. 28 - Describe three important reasons why semisynthetic...Ch. 28 - Imagine yourself as a clinical microbiologist with...
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- How does a rapid test detecting RSV work and what kind of binding does it use (competitve, sandwich, indirect)? Explain using anti-rsv antibody conjugated with gold nanoparticle, anti-rsv antibody, and anti-human IgG. Draw a diagram if possible.arrow_forwardWhat are the different applications of ELISA?arrow_forwardIn ELISA, a substrate is utilized that is cleaved by the enzymatic tag on the antibody. What is this substrate and what color appears when it is cleaved?arrow_forward
- What is the importance of incubating the ELISA plate?arrow_forwardWhat are the two fluorescent compounds that can be used in antibody labelling?arrow_forwardELISA is a vital diagnostic tool. Reagents required for this technique include Thermostable DNA polymerase, RNA primers, and antibodies latex beads, antibodies, glass slide, serum Antigen, Antibodies, microtiter plate, and detection substrate. Thermostable DNA polymerase, DNA primers, and nucleotidesarrow_forward
- Give Explanation Solutionarrow_forwardIs rapid antibody testing a form of ELISA? Explain your answer. How is an ELISA different from rapid antibody testing? Describe how antibodies are used to detect the presence of a specific disease.arrow_forwardCounterstaining with Hematoxylin and Eosin is an important step in which of the following techniques? ELISA Gel Electrophoresis IHC Western blottingarrow_forward
- Explain how labeled antibodies are used in direct and indirect tests.arrow_forwardThere are many immunotherapeutics now being used to immunomodulate recipients that are either fully human monoclonal antibodies or partially humanized monoclonal antibodies. Discuss whether or not they may interfere with solid phase antibody assays, CDC assays or cell based flow cytometry crossmatches.arrow_forwardWhat are the advantages and disadvantages of Nasopharyngeal Swab Test through Reverse-Transcriptase Polymerase Chain Reaction?arrow_forward
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