Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
12th Edition
ISBN: 9780135188996
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
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Chapter 3, Problem 11E.2RQ
Summary Introduction

To determine: The reason why acid-alcohol is used as a decolorizing agent rather than ethyl alcohol.

Introduction: Decolorizing agents are used to establishing color contrast. Decolorizing agents may or may not remove the primary stain from the bacterial cell and it mainly depends on the chemical composition of the cell. Following decolorization, when the primary stain is not washed off, the counterstain cannot penetrate the cell and hence retains the primary stain color.

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