a.
To explain: Why an acid-alcohol as a delocorizing agent, when there is an error is made while performing spore staining.
Introduction: Spore staining is a staining technique that is used to differentiate the bacterial endospores from the vegetative cells. Endospores are formed by a few genera of bacteria, for example, Bacillus. Bacteria are able to thrive in extreme conditions by forming spores. Spores are highly resistant to heat, chemicals, desiccation, and radiation.
b.
To explain: If an error is made while performing spore staining, then what are the microscopic observations that differ from those observed when the slides were prepared correctly. For example, safranin is used as the primary stain and malachite green is used as the counterstain.
c.
To explain: If an error is made while performing spore staining, then what are the microscopic observations that differ from those observed when the slides were prepared correctly. For example, heat is not applied during the primary stain.
Want to see the full answer?
Check out a sample textbook solutionChapter 3 Solutions
Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
- Human Anatomy & Physiology (11th Edition)BiologyISBN:9780134580999Author:Elaine N. Marieb, Katja N. HoehnPublisher:PEARSONBiology 2eBiologyISBN:9781947172517Author:Matthew Douglas, Jung Choi, Mary Ann ClarkPublisher:OpenStaxAnatomy & PhysiologyBiologyISBN:9781259398629Author:McKinley, Michael P., O'loughlin, Valerie Dean, Bidle, Theresa StouterPublisher:Mcgraw Hill Education,
- Molecular Biology of the Cell (Sixth Edition)BiologyISBN:9780815344322Author:Bruce Alberts, Alexander D. Johnson, Julian Lewis, David Morgan, Martin Raff, Keith Roberts, Peter WalterPublisher:W. W. Norton & CompanyLaboratory Manual For Human Anatomy & PhysiologyBiologyISBN:9781260159363Author:Martin, Terry R., Prentice-craver, CynthiaPublisher:McGraw-Hill Publishing Co.Inquiry Into Life (16th Edition)BiologyISBN:9781260231700Author:Sylvia S. Mader, Michael WindelspechtPublisher:McGraw Hill Education