Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
Microbiology: A Laboratory Manual, Loose Leaf Edition (12th Edition)
12th Edition
ISBN: 9780135188996
Author: James G. Cappuccino, Chad T. Welsh
Publisher: PEARSON
Question
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Chapter 3, Problem 12E.3RQ

a.

Summary Introduction

To explain: Why an acid-alcohol as a delocorizing agent, when there is an error is made while performing spore staining.

Introduction: Spore staining is a staining technique that is used to differentiate the bacterial endospores from the vegetative cells. Endospores are formed by a few genera of bacteria, for example, Bacillus. Bacteria are able to thrive in extreme conditions by forming spores. Spores are highly resistant to heat, chemicals, desiccation, and radiation.   

b.

Summary Introduction

To explain: If an error is made while performing spore staining, then what are the microscopic observations that differ from those observed when the slides were prepared correctly. For example, safranin is used as the primary stain and malachite green is used as the counterstain.

c.

Summary Introduction

To explain: If an error is made while performing spore staining, then what are the microscopic observations that differ from those observed when the slides were prepared correctly. For example, heat is not applied during the primary stain.

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