Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed. Concept introduction: Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

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Answer 1

Question

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

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present briefly the principle of size exclusion chromatography. give a brief description of the use of protein assays.

IN A BIURET ASSAY TWO PROTEIN SOLUTION CONTAINING 150MG/ML AND 180MG/ML SHOWS THE SIMILAR ABSORBANCE. IS IT POSSIBLE?

Monoclonal antibodies can be conjugated to an insoluble support by chemical methods. Explain how these antibody-bound beads can be exploited for protein purification.

Answer 2

Question

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

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Answer 3

Question

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

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Answer 4

Question

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

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Answer 5

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

Answer 6

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

Answer 7

Chapter 3, Problem 30P

Interpretation Introduction

Interpretation:

Strategies for the purification of an antibody out of antiserum found after inoculation of antigen needs to be proposed.

Concept introduction:

Biochemists produce antibodies which is specific to certain antigen, by injecting that antigen into a rabbit twice three weeks apart. This antigen stimulates the production of antibodies in the blood which recognize the antigen. Then blood from the rabbit is drawn out and blood serum which contains the antibodies is separated from blood cells from centrifugation. Then antibodies are purified by various strategies for further use because cell may a produce different antibodies for different surface features of the same antigen. These antibodies are referred to as polyclonal antibodies.

Answer 8

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Answer 11

Ch. 3 - Prob. 1P Ch. 3 - Prob. 2P Ch. 3 - Prob. 3P Ch. 3 - Prob. 4P Ch. 3 - Prob. 5P Ch. 3 - Prob. 6P Ch. 3 - Prob. 7P Ch. 3 - Prob. 8P Ch. 3 - Prob. 9P Ch. 3 - Prob. 10P

Solution Summary: The author proposes strategies for the purification of an antibody out of antiserum found after inoculation. These include size exclusion chromatography, ammonium sulphate precipitation, and ion exchange

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