Biochemistry
6th Edition
ISBN: 9781305577206
Author: Reginald H. Garrett, Charles M. Grisham
Publisher: Cengage Learning
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Chapter 31, Problem 4P
Interpretation Introduction
Interpretation:
A rational scenario for chaperone-independent folding of multidomain protein needs to be explained.
Concept Introduction:
Protein folding is known as the folding procedure through which the protein obtains its native 3-D structure, a conformation which is generally function biologically, in a reproducible and expeditious manner.
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resting state of the protein, the Lys 216 Schiff base has pKa = 9.5 and Asp 85 has pKa = 3.5. When the conformational change occurs, the proton that was on the Schiff base moves to Asp 85. This should tell you that one or both of these two pKas
changed with the conformational change. How does the pKa of Asp 85 compare with the pKa of the Lys 216 Schiff base after the conformational change?
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