a)
Interpretation:Reason for streaks of carboxylic acid on silica gel TLC plates should be determined.
Concept introduction:TLC or thin layer chromatography is technique that is used to separate various components of mixture with help of stationary phase and mobile phase. It makes use of silica gel, cellulose or aluminum oxide. This procedure is widely used because of its simplicity, low cost efficiency, and high sensitivity.
b)
Interpretation:Purpose of addition of acetic acid to reduce streaking should be explained.
Concept introduction:TLC or thin layer chromatography is technique that is used to separate various components of mixture with help of stationary phase and mobile phase. It makes use of silica gel, cellulose or aluminum oxide. This procedure is widely used because of its simplicity, low cost efficiency, and high sensitivity.
c)
Interpretation:Substance that can be added to reduce streaks produced by
Concept introduction:TLC or thin layer chromatography is technique that is used to separate various components of mixture with help of stationary phase and mobile phase. It makes use of silica gel, cellulose or aluminum oxide. This procedure is widely used because of its simplicity, low cost efficiency and high sensitivity.
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EBK EXPERIMENTAL ORGANIC CHEMISTRY: A M
- Doe hydrophobic silica gel act as non-polar stationary phase in TLC making the process as reverse chromatography? explainarrow_forwardWhat happens if the spots are made too small when preparing a TLC plate for development?arrow_forwardHow does increasing the polarity of the developing solvent affect the Rf of a compound on a silica gel TLC plate?arrow_forward
- What would happen if we use longer columns and select adsorbing materials with 200-400 mesh sizes for gel filtration chromatography using a column experiment?arrow_forward1. In thin layer chromatography (TLC), why do you need to use a pencil in marking the spots, origin, and solvent front and not a marker? 2. Why is it necessary to mark the solvent front immediately? 3. Why do you need to ensure that the loading spot is above the solvent level in paper chromatography? 4. Why do different compounds travel different distances on the piece of paper?arrow_forwardWhat happens if the spots are made too large when preparing a TLC plate for development?arrow_forward
- Why is sodium chloride added before extraction the aqueous layer with DCM? How does magnesium sulfate break emulsions?arrow_forwarda) Thin layer chromatography (TLC) and high performance liquid chromatography (HPLC) can be used as examples to explain difference between adsorption and partition. For each of these chromatographic techniques, identify: + the stationary and mobile phase + how the mobile phase moves b) How is the Rf value for a spot on a TLC plate calculated? What can the Rf value be used for? In order to achieve the best separation of several components of different polarity in a mixture spotted on a TLC plate, what guideline should one follow regarding the choice of a developing solvent? (i.e. polar, non-polar, etc.)arrow_forwardChromatography is a separation technique. What is the physical or chemical basis for separation of compounds during thin layer chromatography?arrow_forward
- The identity and ratio of solvents are necessary in chromatography for achieving a good separation. What would happen if your solvent had an overly polar TLC developer? What would happen if it was overly non-polar?arrow_forwardWhich statement about TLC analyses is not true? * A- The line at the base of the TLC plate should be drawn with a pencil. B- To prevent contamination, a new capillary tube should be used to apply each sample. C- Samples should be applied multiple times in the same place to increase the concentrations of any possible compounds in the spot. D- A glass beaker lined with filter paper can be used as a development chamber in a TLC analysis. E- All these statements are correct.arrow_forwardIn paper chromatography, a rectangular piece of filter paper serves as the mobile phase stationary phase eluent solute sample spotsarrow_forward
- EBK A SMALL SCALE APPROACH TO ORGANIC LChemistryISBN:9781305446021Author:LampmanPublisher:CENGAGE LEARNING - CONSIGNMENT