BIOCHEMISTRY-ACHIEVE (1 TERM)
9th Edition
ISBN: 9781319402853
Author: BERG
Publisher: MAC HIGHER
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Question
Chapter 35, Problem 10P
Interpretation Introduction
Interpretation:
The purpose of the hybridization of T-cell cDNA with B-Cell mRNA should be determined.
Concept introduction:
The cloning is the technique of production of the gene of desire in another cell. In this, the entire genome or a segment of the genome can be inserted in other cells, where it expresses the desired trait.
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organisms ha
varieties. Through this process, several genetically (10)
been produced.
What I Can Do
Activity 7: MATCHY MATCHY
Direction: Match the purpose to the components found in the box below.
Antibiotic
Resistance Gene
Multiple Cloning Site
Promoter
DNA
Inserted Gene Sequence
Multiple Cloning Site
1. Allows the controlled expression of the desired gene in the
presence of an inducing agent (e.g., beta-galactosidase; heat
treatment (~65°"C)
2. DNA sequence or portion for the insertion of the desired gene.
This section may contain sequences that will be cut by
specific restriction endonucleases ( cuts within the molecule)
3. Successful insertion of a gene allows the expression of its
protein product. This usually provides a specific trait to the
"transformed" bacteria.
4. Provides a way to screen a population of bacteria for those
that took up the plasmid. For example, if an ampicillin
resistance gene is encoded in the plasmid, then only bacteria
10
t4
Gene Expression and the Impact of a Mutation.
Can someone help me to answer the question 8 and 9, please?
8. How has the mutation altered the polypeptide? Is the function of the hemoglobin molecule (which includes 2 ẞ-globin polypeptides and 2 a-globin polypeptides) impaired? (Read your book to learn more about sickle cell disease.)
9. What is the relationship between the genotype in this case and the individual's phenotype?
asap please
Don't copy paste.
Chapter 35 Solutions
BIOCHEMISTRY-ACHIEVE (1 TERM)
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