Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 37, Problem 1AL
ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or false-negative reactions)?
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ELISA tests usually use a primary and secondary antibody. Why? What are the necessary controls one would need to perform to ensure that the antibody specificities are valid (i.e., no false-positive or falsenegative reactions)?
How might concentration of antigen and/or antibody affect the formation of the blue color formation expected. in an ELISA ASSAY?
What are the advantages and disadvantages of agglutinationtests versus fluorescent antibody assays? How are the latter usedto identify specific cells in complex mixtures, such as blood?
Chapter 37 Solutions
Prescott's Microbiology
Ch. 37.2 - Prob. 1MICh. 37.2 - Prob. 2MICh. 37.2 - Prob. 1CCCh. 37.2 - Describe in general how biochemical tests are used...Ch. 37.2 - Prob. 3CCCh. 37.2 - Prob. 4CCCh. 37.2 - Prob. 5CCCh. 37.2 - Prob. 6CCCh. 37.3 - Prob. 1MICh. 37.3 - Prob. 2MI
Ch. 37.3 - Prob. 1CCCh. 37.3 - Prob. 2CCCh. 37.3 - Prob. 3CCCh. 37.3 - Prob. 4CCCh. 37.3 - Prob. 5CCCh. 37.3 - Name two types of immunodiffusion tests and...Ch. 37 - Prob. 1RCCh. 37 - Prob. 2RCCh. 37 - Prob. 3RCCh. 37 - Prob. 4RCCh. 37 - ELISA tests usually use a primary and secondary...Ch. 37 - Prob. 2ALCh. 37 - Prob. 3AL
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- Why do some tests for antibody in serum (such as for HIV and syphilis)require backup verification with additional tests at a later date?arrow_forwardWhat is the effect of not adding the primary antibody to the ELISA reaction?arrow_forwardWhy is a positive and a negative control used in a Elisa experiment?arrow_forward
- what is the principle of ELISA? What is the procedure of direct and indirect ELISA and what is the purpose of each variant in clinical diagnosis?arrow_forwardHow does the formation of a colored product in the wells durin a ELISA ASSAY demonstrate characteristics of antigen-antibody recognition? How might concentration of antigen and/or antibody affect the formation of the blue color formation expected.arrow_forwardWhat does ELISA stand for?arrow_forward
- What is the result of the Antibody Screening Test? Based on the result of the autocontrol, it is safe to say that the antibodies which reacted with the screening cells and panel cells are?arrow_forwardExplain the ELISA protocol and the purpose of each step.arrow_forwardHow you will detect covid-19 using ELISA?arrow_forward
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