Microbiology with Diseases by Body System, Books a la Carte Edition (5th Edition)
5th Edition
ISBN: 9780134618449
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 4, Problem 1CM
Using the following terms, fill in the following concept map that describes
Decolorizer
Ethanol and acetone
Gram-negative bacteria
Gram-positive bacteria
Iodine
Primary stain
Purple
Safranin
Thin peptidoglycan layer
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Complete the following table with your observations from the prepared slide
Name of the
Bacteria
Type of
Endospore
Staining Technique
(+ or −)
Drawing
Clostridium botulinum
Clostridium perfringens
Bacillus anthracis
What is the purpose of each reagent in gram-staining?
Primary stain
Mordant
Decolorizing agent
Counterstain
Gram negative bacteria do not retain the stain when washed with alcohol.Comment.
Chapter 4 Solutions
Microbiology with Diseases by Body System, Books a la Carte Edition (5th Edition)
Ch. 4 - Prob. 1TMWCh. 4 - Why is a Gram-negative bacterium colorless but a...Ch. 4 - Why didnt Linnaeus create taxonomic groups for...Ch. 4 - Necrotizing Fasciitis Fever, chills, nausea,...Ch. 4 - Why is magnification high and color absent in an...Ch. 4 - Prob. 1MCCh. 4 - Prob. 2MCCh. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Prob. 7MCCh. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - In the binomial system at nomenclature, which term...Ch. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Fill in the Blanks 4. ___________ refers to...Ch. 4 - Fill in the Blanks 5. Cationic chromophores such...Ch. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Put the following substances in the order they are...Ch. 4 - Prob. 5SACh. 4 - Prob. 6SACh. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Miki came home from microbiology lab with green...Ch. 4 - Why is the definition of species as successfully...Ch. 4 - With the exception of the discovery of new...Ch. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Microbiologists have announced the discovery of...Ch. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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- Which of the following serves as a counterstain for the gram-stain technique? It also happens to be the counterstain for the endospore staining technique.arrow_forwardList the steps of the gram staining procedure in proper sequence and indicate the color of gram-positive and gram-negative bacteria after each step.arrow_forwardAfter the mordant has been added, Gram-positive organisms are stained __________ and Gram-negative organisms are stained __________. A)purple;pink B)purple;purple C)purple;colorless D) pink;pinkarrow_forward
- The below photograph shows a Gram-stained slide viewed using a light microscope set at brightfield of a clinical sample from a patient with symptoms suggesting an infection. The slide was viewed with x100 objective. What could be the most likely cause of infection? Gram positive bacteria, either rods or cocci Gram positive rod shaped bacteria or yeast Gram negative rod or cocci shaped bacteria Gram negative bacteria, either rods or cocciarrow_forwardDescribe the bacteria observed in different staining procedures. Please refer to the photo - Gram Stain - Acid fast stainarrow_forwardWhich one of the following is the most resistant microorganism for sterilization: Select one: a. Lipid enveloped viruses b. Gram negative bacteria c. Gram positive bacteria d. Mycobacteriumarrow_forward
- When you interpret a Gram-stained smear, you should also describe the morphology (shape) of the cells, and their arrangement. In the figure below, there are two distinct types of bacteria, distinguishable by Gram stain reaction, and also by their shape and arrangement. Below, describe these characteristics for both bacteria: Gram positive bacterium Gram negative bacterium Morphology cocci bacillus Arrangementarrow_forwardWhat is the physical appearance (solid, liquid, semi-solid) of following media:Agar deep tubearrow_forwardAfter performing a Gram stain on a Gram negative bacteria, the specimen does not show pink or purple bacteria. Which of the following could notexplain why this occurred? Decolorizer was used after safranin Safranin was not used Crystal violet was not used The specimen was not heat-fixedarrow_forward
- Create a dichotomous key Use the following bacteria and place them at the top of your key: Escherichia coli Klebsiella pneumoniae Staphylococcus epidermidis Bacillus subtilis Use the following five criteria to create the couplets: Morphology Gram reaction Acid Fast Reaction Endospore Positive/Negative Capsule Positive/Negativearrow_forwardMicroorganism Type of Microorganism Gram Stain Shape Tryptic Soy Agar McConkey Agar Blood Agar EMB Agar Mannitol Salt Agar Bacillus cereus Bacillus megaterium Bacillus subtilis Candida albicans Escherichia coli Micrococcus luteus Neisseria sicca Proteus vulgaris Pseudomonas aeruginosa Serratia marcescens Staphylococcus aureus Staphylococcus epidermidisarrow_forwardYou are performing a Gram stain on Gram-negative bacteria, and you stop after the addition of the alcohol. What is the appearance of the bacteria at this point? ○ pink ○ colorless ○ blue ○ purplearrow_forward
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