BROCK BIOL OF MICRO-ACCESS W/EBOOK
4th Edition
ISBN: 9781323632215
Author: MADIGAN
Publisher: PEARSON C
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Textbook Question
Chapter 4, Problem 2AQ
Compare and contrast the activity of DNA and RNA polymerases. What is the function of each? What are the substrates of each? What is the main difference in the behavior of the two polymerases?
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Draw the reaction between a growing strand of RNA and the next nucleotide added. Clearly show the interaction between the template DNA and the rNTP and draw arrows to indicate the reaction that occurs. How does this compare to what occurs in DNA polymerization?
Describe three major differences between leading and lagging strand synthesis. What feature of DNA polymerase results in the leading and lagging strands being synthesized differently
In what ways are DNA polymerase and RNA polymerase similar? How do they differ?
Chapter 4 Solutions
BROCK BIOL OF MICRO-ACCESS W/EBOOK
Ch. 4.1 - Prob. 1MQCh. 4.1 - Prob. 2MQCh. 4.1 - Prob. 3MQCh. 4.1 - Prob. 4MQCh. 4.2 - Prob. 1MQCh. 4.2 - Prob. 2MQCh. 4.2 - Prob. 3MQCh. 4.2 - Why is supercoiling essential to a bacterial cell?...Ch. 4.3 - Prob. 1MQCh. 4.3 - Prob. 2MQ
Ch. 4.3 - Approximately how large is the Escherichia coli...Ch. 4.3 - Prob. 4MQCh. 4.4 - To which end (5 or 3) of a newly synthesized...Ch. 4.4 - Prob. 2MQCh. 4.5 - Prob. 1MQCh. 4.5 - Prob. 2MQCh. 4.5 - Prob. 3MQCh. 4.6 - Prob. 1MQCh. 4.6 - Prob. 2MQCh. 4.6 - Prob. 3MQCh. 4.7 - Prob. 1MQCh. 4.7 - Prob. 2MQCh. 4.7 - Prob. 3MQCh. 4.7 - Prob. 4MQCh. 4.8 - Prob. 1MQCh. 4.8 - Prob. 2MQCh. 4.8 - Prob. 3MQCh. 4.9 - What three major components make up an archaeal...Ch. 4.9 - Prob. 2MQCh. 4.9 - Prob. 3MQCh. 4.10 - Prob. 1MQCh. 4.10 - Prob. 2MQCh. 4.10 - Prob. 3MQCh. 4.11 - Prob. 1MQCh. 4.11 - Prob. 2MQCh. 4.11 - Prob. 3MQCh. 4.12 - Prob. 1MQCh. 4.12 - What is the function of the acceptor stem of a...Ch. 4.13 - What are the components of a ribosome? What...Ch. 4.13 - How is a completed polypeptide chain released from...Ch. 4.13 - How does tmRNA free stalled ribosomes?Ch. 4.14 - Prob. 1MQCh. 4.14 - Prob. 2MQCh. 4.14 - Prob. 3MQCh. 4.14 - Prob. 4MQCh. 4 - Prob. 1RQCh. 4 - Prob. 2RQCh. 4 - Prob. 3RQCh. 4 - Prob. 4RQCh. 4 - Prob. 5RQCh. 4 - Prob. 6RQCh. 4 - Prob. 7RQCh. 4 - Prob. 8RQCh. 4 - Why are errors in DNA replication so rare? What...Ch. 4 - Prob. 10RQCh. 4 - Prob. 11RQCh. 4 - Prob. 12RQCh. 4 - REVIEW QUESTIONS
13. Why do eukaryotic mRNAs have...Ch. 4 - Prob. 14RQCh. 4 - Prob. 15RQCh. 4 - Prob. 16RQCh. 4 - The enzyme activity that forms peptide bonds on...Ch. 4 - Define the types of protein structure: primary,...Ch. 4 - Prob. 19RQCh. 4 - Prob. 20RQCh. 4 - Prob. 1AQCh. 4 - Compare and contrast the activity of DNA and RNA...Ch. 4 - Prob. 3AQCh. 4 - In Chapter 10 we will learn about mutations,...
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- Explain the molecular mechanism of DNA polymerization by DNA polymerase and explain why DNA polymerase 3 and not dna polymerase 1 is responsible for replicating the bacterial genome.arrow_forwardWhat is the difference between DNA polymerase and RNA polymerase?arrow_forwardDescribe several aspects about RNA polymerases that are unique compared to DNA polymerases.arrow_forward
- It was once thought that the DNA polymerase machinery moves along DNA in a manner analogous to a train on a track. Current evidence indicates that the polymerizing machinery is instead stationary and that the DNA strands are pumped through the complex. What advantages does this stationary mechanism have?arrow_forwardBelow is a study of a colony of cells, determine that some of these cells have a mutated DNA polymerase I that results in loss of function of this enzyme. - What will the effect of the mutation in DNA polymerase I be on DNA replication? Include leading and lagging strand - Will this mutation in DNA polymerase I have an impact on another step in DNA replication? Will DNA be replicatation be impacted? Are any enzymes involved?arrow_forwardCompare and contrast the properties of the enzymes DNA polymerase I and polymerase III from E. coli.arrow_forward
- a) "Out of three E.coli DNA polymerases, DNA polymerases 3 has a high processivity and rate of polymerization and therefore better suited for replication of the genome" What is meant by processivity? how does the DNA polymerase 3 maintain high processivity? b) What is a replication fork ?. Give the protein/enzymes of a replication fork and describe their function?arrow_forwardUsing the figure below identify: What is a function of introns and exons? What is a role of mobile DNA elements? What is a meaning of simple-sequence DNA?arrow_forwardThe following diagram represents a DNA molecule that is undergoing replication. Draw in the strands of newly synthesized DNA and identify (a) the polarity of the newly synthesized strands, (b) the leading and lagging strands, (c) Okazaki fragments, and (d) RNA primers.arrow_forward
- Explain why the active site of poly(A) polymerase is much narrower than that of DNA and RNA polymerases.arrow_forwardWhat factors promote the fidelity of replication during the synthesis of the leading strand of DNA? Would you expect the lagging strand to be made with the same fidelity? Why or why not? Explain your answer briefly.arrow_forwardDoes the addition of a histidine tag affect DNA polymerase activity and or processivity? Give a detailed explanation.arrow_forward
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