Microbiology: With Dis.... - Access (Custom)
4th Edition
ISBN: 9781269937887
Author: BAUMAN
Publisher: PEARSON
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Chapter 4, Problem 5CT
Summary Introduction
To answer:
To find the resolution of two objects at 400nm and 40nm apart by using 100× oil lens.
Introduction:
A microscope is a device used to view the magnified image of an object. There are two types of lens used in the microscope namely, the objective and the ocular lens. The ocular lens has 10× power and the objective lenses have 10×, 40×, and 100× power respectively.
The resolving power of a microscope was increased by using the immersion oil. It was attained by immersing the objective lens and the slide containing the sample in a transparent oil. The 100× objective lens is used for the oil immersion procedure.
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Chapter 4 Solutions
Microbiology: With Dis.... - Access (Custom)
Ch. 4 - Prob. 1TMWCh. 4 - Why is a Gram-negative bacterium colorless but a...Ch. 4 - Why didnt Linnaeus create taxonomic groups for...Ch. 4 - Necrotizing Fasciitis Fever, chills, nausea,...Ch. 4 - Why is magnification high and color absent in an...Ch. 4 - Prob. 1MCCh. 4 - Prob. 2MCCh. 4 - Prob. 3MCCh. 4 - Curved glass lenses _________ light. a. refract b....Ch. 4 - Prob. 5MC
Ch. 4 - Prob. 6MCCh. 4 - Prob. 7MCCh. 4 - Prob. 8MCCh. 4 - Prob. 9MCCh. 4 - In the binomial system at nomenclature, which term...Ch. 4 - Prob. 1FIBCh. 4 - Prob. 2FIBCh. 4 - Prob. 3FIBCh. 4 - Fill in the Blanks 4. ___________ refers to...Ch. 4 - Fill in the Blanks 5. Cationic chromophores such...Ch. 4 - Prob. 1VICh. 4 - Label the microscope.Ch. 4 - Prob. 1SACh. 4 - Critique the following definition of magnification...Ch. 4 - Prob. 3SACh. 4 - Put the following substances in the order they are...Ch. 4 - Prob. 5SACh. 4 - Prob. 6SACh. 4 - Prob. 7SACh. 4 - Prob. 8SACh. 4 - Miki came home from microbiology lab with green...Ch. 4 - Why is the definition of species as successfully...Ch. 4 - With the exception of the discovery of new...Ch. 4 - Prob. 4CTCh. 4 - Prob. 5CTCh. 4 - In what ways are the Gram stain and the acid-fast...Ch. 4 - Prob. 7CTCh. 4 - Prob. 8CTCh. 4 - Why is the genus name Coccus placed within...Ch. 4 - A clinician obtains a specimen of urine from a...Ch. 4 - Using the following terms, fill in the following...
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- Why should you always begin to use a microscope with a low-power objective?arrow_forwardI have a microscope with oculars that are 20x and am viewing a slide using oculars that are 50x. What is the overall magnification?arrow_forwardWhat are the corresponding ring colors of the objective lenses of the microscope? Scanner _________________________ Low Power Objective (LPO) _________________________ High Power Objective (HPO) _________________________ Oil immersion Objective (OIO) _________________________arrow_forward
- Using a good compound light microscope with a resolving power of 0.3 µm, 10X ocular lens, and a 100X oil immersion lens. Would you be able to discern two objects separated by 3 µm? 0.3 µm? 300 nm?arrow_forwardWhat is the final magnification that you would obtain if you were viewing an object through a microscope with a 40X objective lens and a 25.5X ocular lens?arrow_forwardThe stereoscopic microscope offers a large____________between the objective lens and the specimen.arrow_forward
- Explain when to use bright-field, phase-contrast, dark-field, fluorescence, transmission electron, and scanning electron microscopy for a given situation. What is an example of this situation?arrow_forwardRowena was tasked to measure the actual magnification of her specimen mounted on a slide without drawing or taking a photomicrograph. Which type of microscope should she use? Why? How does it help her get the actual magnification?arrow_forwarda. Do you open both eyes or close one eye when viewing a specimen under a microscope? Why? Why not? b. In using a compound microscope, do you start with the low power objective (LPO) or the high power objective (HPO)? Why or Why not? c. When using 60X, do you it together with the coarse adjustment knob or fine adjustment knob? d. Why must you slowly turn the fine adjustment knob? e. Is natural light or is artificial light used when using the microscope?arrow_forward
- If you are using a microscope and a specimen takes up 1/4 of the field of vview at 1000X, what is the diameter?arrow_forwardWhy did we calculate, rather than measure, the diameter of the field of view on medium and high power? When using the compound microscope, you always begin viewing your specimen on __________ power, and use the __________ focus knob.arrow_forward
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