SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
9th Edition
ISBN: 9781319398583
Author: BERG
Publisher: MAC HIGHER
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Question
Chapter 5, Problem 12P
Interpretation Introduction
Interpretation:
The affect of altering temperature of hybridization on the PCR amplification is to be stated. The way by which the controlling of stringency of the hybridization helps an individual is to be stated.
Concept introduction:
DNA stands for deoxyribonucleic acid, is a biological macromolecule. DNA contains double helical strands along with the complementary base pairs. The four complementary bases of DNA are adenine (A), thymine (T), guanine (G) and cytosine (C).
The reaction that is used to make the duplicates of a particular DNA segment is known polymerase chain reaction (PCR).
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This is DWA.
You hope to clone an extinct animal species by taking the easy route - using museum bones or
tissues. You extract the DNA and use PCR to amplify, then sequence all segments of the genome
You are unsuccessful. Later you discover that the museum specimens have been treated with
formaldehyde, which forms covalent bonds in DNA, where once there existed H-bonds.
What step in your PCR reaction would be inhibited?
g
-S
Knowing this, if a human was exposed to formaldehyde, what enzyme(s) of DNA
replication in vivo would be prevented from doing their job?
organisms ha
varieties. Through this process, several genetically (10)
been produced.
What I Can Do
Activity 7: MATCHY MATCHY
Direction: Match the purpose to the components found in the box below.
Antibiotic
Resistance Gene
Multiple Cloning Site
Promoter
DNA
Inserted Gene Sequence
Multiple Cloning Site
1. Allows the controlled expression of the desired gene in the
presence of an inducing agent (e.g., beta-galactosidase; heat
treatment (~65°"C)
2. DNA sequence or portion for the insertion of the desired gene.
This section may contain sequences that will be cut by
specific restriction endonucleases ( cuts within the molecule)
3. Successful insertion of a gene allows the expression of its
protein product. This usually provides a specific trait to the
"transformed" bacteria.
4. Provides a way to screen a population of bacteria for those
that took up the plasmid. For example, if an ampicillin
resistance gene is encoded in the plasmid, then only bacteria
10
t4
Chapter 5 Solutions
SAPLINGPLUS F/BIOCHEM+ICLICKER REEF-CODE
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