EBK MICROBIOLOGY:W/DISEASES BY BODY...-
5th Edition
ISBN: 9780134608242
Author: BAUMAN
Publisher: PEARSON
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Textbook Question
Chapter 5, Problem 22CT
Suppose you could insert a tiny pH probe into the space between mitochondrial membranes. Would the pH be above or below 7.0? Why?
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If protein X produces a higher absorbance reading at pH 7 than it does at pH 9, what can you conclude?
Question 1 options:
A lesser amount of protein X was used in the reaction at pH 7 than at pH 9.
A greater amount of protein X was used in the reaction at pH 9 than at pH 7.
Protein X is a more efficient catalyst at pH 7 than it is at pH 9.
Protein X is a more efficient catalyst at pH 9 than it is at pH 7.
You wish to separate and purify enzyme A from contaminating enzymes B and C. Enzyme A is found in the matrix of the mitochondria. Enzyme B is embedded in the mitochondrial membrane, and enzyme C is found in the peroxisome. Enzymes A and B have molecular weights of 60,000 Da. Enzyme C has a molecular weight of 100,000 Da. Enzyme A has a pI of 6.5. Enzymes B and C have pI values of 7.5. Design an experiment to separate enzyme A from the other two enzymes.
Suppose you wish to sediment a sample of spherical protein molecules (molar mass
of 98.1 kg/mol and radius 3.40x10-9 m) in a liquid using a centrifuge. The sample is
placed 5.0 cm from the centrifuge's axis of rotation and is rotated at 6000 Hz.
a) What is the centripetal acceleration (i.e. the "g") the sample experiences from the
centrifuge?
b) If the liquid has a density of 900 kg/m3 and the terminal velocity of the protein is
4.10x10 6 m/s, what is the viscosity of the liquid?
Chapter 5 Solutions
EBK MICROBIOLOGY:W/DISEASES BY BODY...-
Ch. 5 - How can oxidation take place in an anaerobic...Ch. 5 - Why do electrons carried by NADH allow for...Ch. 5 - Why does catabolism of amino acids for energy...Ch. 5 - An uninformed student describes the Calvin-Benson...Ch. 5 - Prob. 5TMWCh. 5 - Why is feedback inhibition necessary for...Ch. 5 - Breaks a large molecule into smaller ones a....Ch. 5 - Includes dehydration synthesis reactions a....Ch. 5 - Prob. 3MCCh. 5 - Prob. 4MC
Ch. 5 - Involves the production of cell membrane...Ch. 5 - Includes hydrolytic reactions a. anabolism only b....Ch. 5 - Includes metabolism a. anabolism only b. both...Ch. 5 - Prob. 8MCCh. 5 - A reduced molecule _________. a. has gained...Ch. 5 - Prob. 10MCCh. 5 - Coenzymes are ________. a. types of apoenzymes b....Ch. 5 - Which of the following statements best describes...Ch. 5 - Which of the following does not affect the...Ch. 5 - Most oxidation reactions in bacteria involve the...Ch. 5 - Under ideal conditions, the fermentation of one...Ch. 5 - Under ideal conditions, the complete aerobic...Ch. 5 - Which of the following statements about the...Ch. 5 - Reactions involved in the light-independent...Ch. 5 - The glycolysis pathway is basically __________. a....Ch. 5 - A major difference between anaerobic respiration...Ch. 5 - 1. _______ Occurs when energy from a compound...Ch. 5 - Fill in the Blanks 1. The final electron acceptor...Ch. 5 - Fill in the Blanks 2. Two ATP molecules are used...Ch. 5 - Fill in the Blanks 3. The initial catabolism of...Ch. 5 - Fill in the Blanks 4. ________ is a cyclic series...Ch. 5 - Fill in the Blanks 5. The final electron acceptor...Ch. 5 - Fill in the Blanks 6. Three common inorganic...Ch. 5 - Fill in the Blanks 7. Anaerobic respiration...Ch. 5 - Fill in the Blanks 8. Complete the following...Ch. 5 - Prob. 9FIBCh. 5 - Fill in the Blanks 10 The main coenzymes that...Ch. 5 - VISUALIZE IT! 1 Label the mitochondrion to...Ch. 5 - Label the diagram below to indicate acetyl-CoA,...Ch. 5 - Examine the biosynthetic pathway for the...Ch. 5 - Prob. 1SACh. 5 - Why we enzymes necessary for anabolic reactions to...Ch. 5 - How do organisms control the rate of metabolic...Ch. 5 - How does a nor-competitive inhibitor at a single...Ch. 5 - Explain the mechanism of negative feedback with...Ch. 5 - Facultative anaerobes can live under either...Ch. 5 - How does oxidation of a molecule occur without...Ch. 5 - List at least four groups of microorganisms that...Ch. 5 - Why do we breathe oxygen and give of carbon...Ch. 5 - Why do cyanobacteria and algae take in carbon...Ch. 5 - What happens to the carbon atoms in sugar...Ch. 5 - How do yeast cells make alcohol and cause bread to...Ch. 5 - Where specifically does the most significant...Ch. 5 - Why are vitamins essential metabolic factors for...Ch. 5 - A laboratory scientist notices that a cer1ain...Ch. 5 - Arsenic is a poison that exists in two states in...Ch. 5 - Explain why an excess of all three of the amino...Ch. 5 - Why might an organism that uses glycolysis and the...Ch. 5 - Describe how bacterial fermentation causes milk to...Ch. 5 - Giardia intestinalis and Entamoeba histolytica are...Ch. 5 - Two cultures of a facultative anaerobe are grown...Ch. 5 - What is the maximum number of molecules of ATP...Ch. 5 - In terms of its effects on human metabolism, why...Ch. 5 - Cyanide is a potent poison because it irreversibly...Ch. 5 - How are photophosphorylation and oxidative...Ch. 5 - Members of the pathogenic bacterial genus...Ch. 5 - Compare and contrast aerobic respiration,...Ch. 5 - Scientists estimate that up to one-third of Earths...Ch. 5 - A young student was troubled by the idea that a...Ch. 5 - If a bacterium uses beta-oxidation to catabolize a...Ch. 5 - Some desert rodents rarely have water to drink....Ch. 5 - Prob. 17CTCh. 5 - We have examined the total ATP, NADH, and FADH2...Ch. 5 - Explain why hyperthermophiles do not cause disease...Ch. 5 - In addition to extremes in temperature and pH,...Ch. 5 - Figure 5.18b illustrates events in aerobic...Ch. 5 - Suppose you could insert a tiny pH probe into the...Ch. 5 - Even though Pseudomonas aeruginosa and...Ch. 5 - Photosynthetic organisms are rarely pathogenic....Ch. 5 - Prob. 25CTCh. 5 - A scientist moves a green plant grown in sunlight...Ch. 5 - What class of enzyme is involved in amination...Ch. 5 - Using the following terms, fill in the following...
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