Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 5, Problem 8MC
Aseptically boxed juices and cream containers are processed using which of the following heating methods?
a) Commercial canning
b) High-temperature-short-time (HTST) method
c) Autoclaving
d) Ultra-high-temperature (UHT) method
e) Boiling
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Given the following common laboratory materials, which of the three methods of heat sterilization do you think is the most appropriate and practical to use in sterilizing them ? Why do you think so ? a) empty flask b)L-rod (glass rod c)10 ml glass pipet d) wire needles e)antibiotic solution
1.) During a laboratory activity, Chemical reactions from mixture of chemicals should be observed under which laboratory equipment?
a.) autoclave
b.) fume hood
c.) Labaratory Safety cabinet
d.) Centrifuge
2.) Which type of equipment contains a HEPA filter that only removes suspended materials from exhausted air?
a.) Fume hood
b.) Biosafety Cabinet I
c.) Biosafety Cabinet II
d.) Biosafety Cabinet III
6) The autoclave desired Temperature to kill bacterial spores must be left for at least ____ in order to effectively kill the bacterial spores: a) 5 mins b) 10 mins c) 15 mins d) ½ hr e) 1 hr.7) The autoclave pressure must reach at least ________ in order to effectively kill the bacterial spores: a)15 PSI b) 15 Joules c) 15 KSI d) 15 Lbs e) 15 Atmospheres8) The antibiotic that inhibits the cell wall formation through the -Lactam ring is/are:a) Vancomycin b) Tetracycline c) Penicillin d) Cephalosporin e) c & d9) The sum of all chemical reactions in your body defines: a) anabolism b) metabolism c) catabolism d) Cellular Respiration e) Biosynthesis10) If in a bacterial growth medium waste product accumulates than the bacterial growth stage will be called: a) Death b) Convalescence c) Lag phase d) Log e) Stationary
Chapter 5 Solutions
Nester's Microbiology: A Human Perspective
Ch. 5 - How is preservation different from pasteurization?Ch. 5 -
2. What is the most chemically resistant...Ch. 5 - Explain why it takes longer to kill a population...Ch. 5 - What is the primary reason that wine is...Ch. 5 -
5. What is the primary reason that milk is...Ch. 5 - When canning, why are low-acid foods processed at...Ch. 5 - How are heat-sensitive liquids sterilized?Ch. 5 -
8. How does microwaving a food product kill...Ch. 5 -
9. How is an iodophore different from a tincture...Ch. 5 - Name two products commonly sterilized using...
Ch. 5 - Unlike a disinfectant, an antiseptic a) sanitizes...Ch. 5 - The D value is defined as the time it takes to...Ch. 5 - Prob. 3MCCh. 5 - Prob. 4MCCh. 5 - Which concentration of alcohol is the most...Ch. 5 - All of the following could be used to sterilize an...Ch. 5 - All of the following are routinely used to...Ch. 5 - Aseptically boxed juices and cream containers are...Ch. 5 - Commercial canning processes are designed to...Ch. 5 -
10. Which of the following is false?
a) A...Ch. 5 - An agriculture extension agent is preparing...Ch. 5 - As a microbiologist representing a food...Ch. 5 - This graph shows the time it takes to kill...Ch. 5 -
2. This diagram shows the filter paper method...
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- 11. Foods can be preserved in all the followings except: a) lyophilizing b) Drying c) Salting d) Fermentation 12. The temperature of the autoclave should be held for _____ minutes at least: a) 10 b) 15 c) 20 d) 30 13. The organism used to produce soft cheese is: a) Penicillium b) lactic acid producing bacteria c) ethanol producing bacteria d) a and carrow_forwardListen Which of the following methods of microbial control is used most often with surgical equipment? a) autoclave b) sugar and salting c) dessication d) lyophilizationarrow_forwardAccording to USP Chapter <797>, allergenic extract preparation can be made in a doctor’s office facility by trained personnel only if the allergenic extract preparation: a)Is compounded under a laminar air flow hood Is compounded by a pharmacist b)Contains an antimicrobial preservative c)Is used immediately after preparation d)Is prepared only from plant origin materialarrow_forward
- Choose two from the following culture preservations that are best to use when only an ordinary refrigerator is available for storage: a) Drying b) Mineral oil overlay c) Freezing with liquid nitrogen d) Lyophilization e) Periodic transfer to fresh mediumarrow_forwardWhich concentration of alcohol is the most effective germicide?a) 100% b) 75% c) 50%d) 25% e) 5%arrow_forwardProvide the conditions (e.g., intrinsic properties, storage conditions, etc.) that will make the recovery of the following microorganisms in commercially sterile foods acceptable: a) Obligate thermophilic sporeforming bacteria b) Acid-tolerant microorganisms c) Mesophilic or thermophilic sporeformersarrow_forward
- The thermal death point is best described as:(a) The highest temperature that will support growth of amicrobial culture(b) The length of time required for killing 100% of mi-crobes in a 24-hour culture that is heated to 100°C(c) The temperature at which a previously growing 24-hourculture will begin to die(d) The temperature at which 100% of the microbes in a24-hour culture will be killed in 10 minutesarrow_forwardThe Petri Dish method is used in microbiology to raise bacteria in: a) rapid growth b) pure culture c) septic environment d) all of the above 2. What is the difference between antiseptic and sanitization?3. In order to prevent any kind of contamination the medium must be _________ before placing it in the Petri dish. a) lyophilized b) pasteurized c) autoclaved d) distillearrow_forwardMueller-Hinton agar needs to be made to specific standards in order to yield reliable, reproducible results. How deep is the agar on a Mueller-Hinton agar plate? a) 2 mm b) 4 mm c) 6 mm d) 8 mmarrow_forward
- 5. The autoclave raises the atmospheric pressure to _____ : a) 10 psi b) 15 psi c) 20 k joules d) 25 watts 6. In the experiment with serial dilutions, the plates which should be used for the final count for colonies should have a range of _______ CFU in order to have a reliable estimate: a) <10 b)10-15 c) 15-20 d) none of the above 7. The temperature to incubate the bacteria in the above mentioned experiment should be __: a) 22 oC b) 37 oC c) 98 oC d) 37 oarrow_forwardBased on the knowledge acquired about the conservation and propagation of microorganisms, propose methodologies for:a) conservation of stock culture (used infrequently) of baker's yeast (S. cerevisiae);b) conservation of the working culture (used daily) of the baker's yeast;c) activation (1st stage of propagation) of the work culture;d) propagation of cultures until inoculating a 100 L reactor toi) production of baker's yeast;ii) ethanol production.arrow_forwardIn sterilization, which among the supplies, instruments, glassware, etc. under the list of materials can be sterilized using either or both equipment below? List them down under the category: a) For “autoclaving” only, B) For Dry heat oven sterilization ,And C) Can be sterilized with either. Materials: 200-ml Erlenmeyer flask Stove 500-ml Erlenmeyer flask Autoclave 10-mL graduated cylinder Analytical balance 100-ml graduated cylinder pH meter Spatula Stirring rod 100-mL beaker Test tubes Distilled water Petri dish Stirring rod Alcohol lamp Glass dropperarrow_forward
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