Chapter 6, Problem 10RTC

Summary Introduction

To analyze:

Essential features of recombinant plasmids that are required for the expression of a foreign gene. How can foreign proteins be modified to facilitate its purification? The advantage of expressing a foreign protein in mammalian cells versus bacterial cells.

Introduction:

A recombinant expression plasmid is the one that allows the translation of a gene when introduced into a vector. A recombinant cloning plasmid on the other hand contain the DNA fragment but is unable to produce protein for that gene.