Molecular Cell Biology
Molecular Cell Biology
8th Edition
ISBN: 9781464183393
Author: Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Anthony Bretscher, Hidde Ploegh, Angelika Amon, Kelsey C. Martin
Publisher: W. H. Freeman
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Chapter 6, Problem 8RTC
Summary Introduction

To write:

Three steps involved in each cycle of a PCR reaction and importance of the discovery of a thermostable DNA polymerase for the development of PCR.

Introduction:

PCR (polymerase chain reaction) is a technique, used for the in vitro amplifying desired DNA (deoxyribonucleic acid) molecules. It involves three steps, during which, double-stranded DNA first get separated, joins with primer, and nucleotides are added to the daughter strand. Discovery of a heat-stable DNA polymerase was important for the development of PCR as it does not get denatured at high temperature required for PCR.

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