Brock Biology of Microorganisms Plus MasteringMicrobiology with eText -- Access Card Package (14th Edition)
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Chapter 6, Problem 1AQ

Apart from genome size, what factors make complete assembly of a eukaryotic genome more difficult than assembly of a genome from a species of Bacteria or Archaea?

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Why do geneticists studying eukaryotic organisms often construct cDNA libraries, whereas geneticistsstudying bacteria almost never do? Why might bacterial geneticists have difficulties constructing cDNA libraries even if they wanted to?
What is the main reason for using a cDNA library rather than a genomic library to isolate a human gene from which you wish to make large quantities of the human protein in bacteria? How will you identify clones of interest?
What are some reasons why, in multicellular eukaryotes, genome size is not necessarily related to number of protein-coding genes or organismal complexity?

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Brock Biology of Microorganisms Plus MasteringMicrobiology with eText -- Access Card Package (14th Edition)

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Genome Annotation, Sequence Conventions and Reading Frames; Author: Loren Launen;https://www.youtube.com/watch?v=MWvYgGyqVys;License: Standard Youtube License