Concept explainers
Interpretation:
The alignment that has a higher score according to identity-based alignment scoring.
Concept introduction:
Identity−based alignment scoring is a method to score the identical alignments present in a sequence. The identical amino acids are added while the gaps in the sequence are subtracted to determine the identical score.
(b)
Interpretation:
The alignment that has a higher score according to the Blosum -62 substitution method.
Concept introduction:
Blosum-62 is a type of substitution matrix that is used to score the replacement of amino acids. A positive score of substitution matrix indicates that substitution of amino acid occurs more frequently while a negative score is rare for substitutions.
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- What is the length in AA’s of the LilP protein? Assume fMet is NOT CLEAVED. Enter just the number, nothing else! Write out the sequence of the polypeptide in AA: use the three letter notation, e.g. Met-Ser-Pro- A lilP mutant called lilPXS is isolated that produces a truncated polypeptide of only 6 AA in length. Describe a single basepair DNA change that would lead to this truncated version of the protein. Multiple options are possible (100 words max.)arrow_forwardCompute the percent identity of the following pairwise sequence alignment: -TGAGACTTAGAGT |..|... | | | | | ATAGGAGCGAGAGTarrow_forwardCalculate the dynamic programming matrix and the optimal local and global alignment for the DNA sequences a: GAATTC and b: GATTA, scoring +2 for a match, -1 for a mismatch, and using a linear gap penalty function WL) = -2L thank youarrow_forward
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