Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 9R
DRAW IT Draw the following growth curves for E. coli, starting with 100 cells with a generation time of 30 minutes at 35°C, 60 minutes at 20°C, and 3 hours at 5°C.
- a. The cells are incubated for 5 hours at 35°C.
- b. After 5 hours, the temperature is changed to 20°C for 2 hours.
- c. After 5 hours at 35°C, the temperature is changed to 5°C for 2 hours followed by 35°C for 5 hours.
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Draw the following growth curves for E. coli, starting with 100 cells with a generation time of 30 minutes at 35°C 60 minutes at 20°C, and 3 hours at 5°C. The cells are incubated for 5 hours at 35°C
Based on the growth curve, which ONE of the following best describes what would happen if sample of culture in the presence of drug B is inoculated onto drug-free growth medium?
Select one:
A.
Growth present, but cell count increased by at least 99.9% of the original culture
B.
No growth after 24 hrs
C.
Growth at 24 hrs
D.
Not possible to predict because the culture could either be alive or dead
E.
Growth present, but cell count reduced by at least 99.9% of the original culture
Suppose that you were tasked with 4 cultures of a specific Bacteria Species in Luria Broth medium: (1) culture A – cells are in lag phase; (2) culture B – cell in log phase; (3) culture C – cells in stationary phase; and culture D – cells in decline phase. Imagine that you observe the growth rates of each culture in a fresh sterilized LB medium. Now, plot the outcome growth curves of cultures A, B, C and D in a single graph.
Chapter 6 Solutions
Microbiology: An Introduction (13th Edition)
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