Microbiology: An Introduction (13th Edition)
13th Edition
ISBN: 9780134605180
Author: Gerard J. Tortora, Berdell R. Funke, Christine L. Case, Derek Weber, Warner Bair
Publisher: PEARSON
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Textbook Question
Chapter 6, Problem 6R
A pastry chef accidentally inoculated a cream pie with six S. aureus cells. If S. aureus has a generation time of 60 minutes, how many cells would be in the cream pie after 7 hours?
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Chapter 6 Solutions
Microbiology: An Introduction (13th Edition)
Ch. 6 - Prob. 1RCh. 6 - Prob. 2RCh. 6 - Prob. 3RCh. 6 - Seven methods of measuring microbial growth were...Ch. 6 - By deep-freezing, bacteria can be stored without...Ch. 6 - A pastry chef accidentally inoculated a cream pie...Ch. 6 - Nitrogen and phosphorus added to beaches following...Ch. 6 - Differentiate complex and chemically defined...Ch. 6 - DRAW IT Draw the following growth curves for E....Ch. 6 - NAME IT A prokaryotic cell hitched a ride to Earth...
Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Two culture media were inoculated with four...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Use the following graph to answer questions 3 and...Ch. 6 - Assume you inoculated 100 facultatively anaerobic...Ch. 6 - The term trace elements refers to a. the elements...Ch. 6 - Which one of the following temperatures would most...Ch. 6 - Which of the following is not a characteristic of...Ch. 6 - Which of the following types of media would not be...Ch. 6 - An organism that has peroxidase and superoxide...Ch. 6 - E. coli was incubated with aeration in a nutrient...Ch. 6 - Clostridium and Streptococcus are both...Ch. 6 - Most laboratory media contain a fermentable...Ch. 6 - Flask A contains yeast cells in glucose-minimal...Ch. 6 - Clinical Applications and Evaluation 1. Assume...Ch. 6 - Heat lamps are commonly used to maintain foods at...Ch. 6 - The number of bacteria in saliva samples was...
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- a. If an egg salad sandwich sitting in a warm car for 4 hours develops 40,960bacterial cells, how many more cells would result in just one more generation? b. What would the cell count be after 4 hours if the initial bacterial dose was 100?c. What do your answers tell you about using clean techniques in food preparation and storage (other than esthetic considerations)?arrow_forwardIf the original number of cells in a sample was 12,000, how many bacteria will be present in 12 hours when the generation time is 15 minutes?arrow_forwardIf a flask is inoculate with 10 E. coli cells. After 6 hours you now have 200,000 cells. determine both n (number of generations) and g (generation time) in minutes? A culture of S. epidermidis is growing. It divides every 20 min. After 4 hours, how many generations occurred?arrow_forward
- Disinfectants, be they heat or radiation or chemicals, usually kill a constant proportion of the cells present per unit time if the cells are all equally susceptible. Hence, the term ‘log kill.’ The probability of death is constant over time given a ‘single hit.’ The more cells present at the start, the greater the number killed per unit time and the longer it will take to kill them ‘all.’ One decimal reduction is one log place (exponent). Log Nt = Log N0 – t/D. D is the time required for a log kill. A suspension of 105 CFU/mL of Salmonella is treated with bleach, and 90% of the cells are dead after 10 minutes. How many viable cells per mL would you expect after 30 minutes? Yes, the 30 minutes here implies 3 logs of killing. This is an excellent problem to use with the Death Equation because you can reason it through first, and then solve it using the equation.arrow_forwardA food sample was cooked. The bacterial population in the food decreased from 500,000 cells to 5 cells in 30 minutes. What is the decimal reduction time for this treatment?arrow_forwardHow long would it take a single bacterial cell to form 1,000,000 cells if it had a generation time of 30 minutes?arrow_forward
- Why would you want to limit the number of times you passage a cell culture line?arrow_forwardA 73 year old woman is admitted to hospital for treatment of an abscess from staph. It was necessary to disinfect the hospital room after her discharge. One thousand of S. aureus cells are exposed to disinfectant. After 10 minutes, 90% of the cells are killed. How many cells are viable after 20 minutes?arrow_forwardDraw the following growth curves for E. coli, starting with 100 cells with a generation time of 30 minutes at 35°C 60 minutes at 20°C, and 3 hours at 5°C. The cells are incubated for 5 hours at 35°Carrow_forward
- A slant is inoculated with 100 E.coli cells. The slant is left at room temperature where the generation time is 1 hour. How many E. coli are on the slant in 6 hours? If the slant was initially put in the 37C incubator, with a 20 minute generation time, how many more cells would have grown in the same time period?arrow_forwardDuring death phase, microbial cells are...arrow_forwardA 23 year old woman has 10 E coli in her bladder. The organism has generation time of 20 minutes. After a lag of 20 minutes, the E. coli entered the log phase of growth. After 3 hours, what is the total number of cells?arrow_forward
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