The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Question

Want to see the full answer?

Answer 1

Question

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Students have asked these similar questions

a. Let’s say for example that a milk sample has 10,000 bacteria per milliliters. If 1 mL of this sample were plated out, these would theoretically be 10,000 colonies in the Petri plate. Discuss and explain the serial dilutions of this example. b. The disk diffusion method was used to evaluate 3 disinfectants. The results were as follows: Solution Zone of inhibition X 0 mm Y 5 mm Z 10 mm How would you interpret? Provide inference.

A pure bacterial culture of unknown concentration was diluted to determine the concentration of viable bacteria in the original culture. Serial dilutions were performed as 1. diagrammed below. Each dilution tube contained 400 ul of diluent and 100 ul was transferred into each tube. TSA plates were inoculated with 100 µul from the last three dilution tubes. a. What is the dilution between each tube shown in the diagram below? Express your answer as a ratio. b. What is the total dilution of tube number 5? Express your answer as a ratio. c. What is the concentration of viable bacteria in the original culture? Express your answer using scientific notation and the units CFU/ml. d. What is the concentration of viable bacteria in tube number 2? Express your answer using the units CFU/ml. e. If you inoculated a TSA plate with 250 µl from tube number 5, how many colonies would you expect to see after the plate was incubated? 1 2 3 5 423 80 13 Number of colonies

You spread 0.1 mL volume of a 10^(-6) dilution onto a nutrient agar plate. After 24 hours of incubation at 37°C, there were 280 colonies of bacteria on the plate. A.) What is the original concentration (OCD) of bacteria in the stock sample this dilution came from? B.) Using the OCD value from part A, determine the number of colonies that would be expected to grow on a plate that is inoculated with 0.1 mL volume of 10^(-8) dilution from this same stock of bacteria. Show your work for both.

Answer 2

Question

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 3

Question

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 4

Question

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Expert Solution & Answer

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 5

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Answer 6

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Answer 7

Chapter 7, Problem 1A

Summary Introduction

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Answer 8

Expert Solution & Answer

Answer 9

Expert Solution & Answer

Answer 10

Want to see the full answer?

Check out a sample textbook solution

See solution

Answer 11

Ch. 7 - The thermal death time for a suspension of...Ch. 7 - If pasteurization does not achieve sterilization,...Ch. 7 - Thermal death point is not considered an accurate...Ch. 7 - Prob. 4R Ch. 7 - DRAW IT A bacterial culture was in log phase in...Ch. 7 - How do autoclaving, hot air, and pasteurization...Ch. 7 - How do salts and sugars preserve foods? Why are...Ch. 7 - Prob. 8R Ch. 7 - A large hospital washes burn patients in a...Ch. 7 - Prob. 10R

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Solution Summary: The author describes the disk-diffusion method used to evaluate the efficacy of a chemical agent or to test the antibiotic sensitivity of bacteria.

Concept explainers

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Want to see the full answer?

Chapter 7 Solutions

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Solution Summary: The author describes the disk-diffusion method used to evaluate the efficacy of a chemical agent or to test the antibiotic sensitivity of bacteria.

Concept explainers

Introduction: The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.

Want to see the full answer?

Chapter 7 Solutions

Introduction:

The disk-diffusion method tests the effectiveness of antibiotics on a specific microorganism.