Campbell Biology in Focus
Campbell Biology in Focus
3rd Edition
ISBN: 9780134710679
Author: Lisa A. Urry, Michael L. Cain, Steven A. Wasserman, Peter V. Minorsky, Rebecca Orr
Publisher: PEARSON
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Chapter 8, Problem 10TYU

DRAW IT The following diagram represents an experiment with isolated thylakoids. The thylakoids were first made acidic by soaking them in a solution at pH 4. After the thylakoid space reached pH 4, the thylakoids were transferred to a basic solution at pH 8. The thylakoids then made ATP in the dark. (See Concept 2.51&1 to review pH.)

Chapter 8, Problem 10TYU, DRAW IT The following diagram represents an experiment with isolated thylakoids. The thylakoids were

Draw an enlargement of part of the thylakoid membrane in the beaker with the solution at pH 8. Draw ATP synthase. Label the areas of high H· concentration and low W concentration. Show the direction protons flow through the enzyme, and show the reaction where ATP is synthesized. Would ATP end up in the thylakoid or outside of it? Explain why the thylakoids in the experiment were able to make ATP in the dark.

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Complete an enlargement of part of the thylakoid membrane in the beaker with the solution at pH 8. Label the areas of high H+ concentration and low H+ concentration. Show the reaction where ATP is synthesized. Drag the appropriate labels to their respective targets. Labels can be used once, more than once, or not at all.
Thylakoids were isolated from chloroplasts and incubated in the dark in an acidic solution (pH 4) to equilibrate the pH.  After 30 minutes, the thylakoids were transferred to a basic solution (pH 8) and kept in the dark.  Will this system produce ATP?  Explain.  Will this system produce G3P?  Explain.
what is the mechanism driving the bromothymol blue solution color change? This is a quesiton from my bio lab as how cellular respiration occurs in germinated beans, dry beans, or no beans at all. Anyone know?
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