ESSENTIALS OF GENETICS-MODIFIED ACCESS
9th Edition
ISBN: 9780134190006
Author: KLUG
Publisher: PEARSON
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Chapter 8, Problem 19PDQ
If a single bacteriophage infects one E. coli cell present in a culture of bacteria and, upon lysis, yields 200 viable viruses, how many phages will exist in a single plaque if three more lytic cycles occur?
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If a single bacteriophage infects one E. coli cell present in a cultureof bacteria and, upon lysis, yields 200 viable viruses, howmany phages will exist in a single plaque if three more lytic cyclesoccur?
1 mL of a bacteriophage suspension is mixed with 20 mL of a bacterial culture and 50% of the phages adsorb. We know that the bacteriophage suspension had a concentration of 1x1010 viruses per mL, and the bacterial culture had a concentration of 3x108 bacteria per mL.
What fraction of the cells is multiple infected?
When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?
Chapter 8 Solutions
ESSENTIALS OF GENETICS-MODIFIED ACCESS
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- Describe in general terms how bacteriophage lambda regulates the switch between lytic and lysogenic cyclesarrow_forwardHow many bacteriophage particles were isolated from a single plaque? Howmany different strains of phage would be present?arrow_forwardOne mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. What fraction of the cells is uninfected?arrow_forward
- One mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. What fraction of the cells is single infected?arrow_forwardWhat enzyme is responsible for the production of viral DNA from a retrovirus?arrow_forwardIn a petri dish with solidified agar with escherichia coli, enterobacter aerogenes and staphylococcus aureus, you streak a loopfull of lytic T4-phage in a single line onto the center of the the dish, how do you know if bacteriophage infected the bacteria. why didn't the bacteriophage infect all 3 bacteria?arrow_forward
- One mL of a bacteriophage suspension is mixed with 20 mL of a bacterialculture and 50% of the phages adsorb. We know that the bacteriophagesuspension had a concentration of 1x10^10 viruses per mL, and the bacterialculture had a concentration of 3x10^8 bacteria per mL. How many viruses are adsorbed per cell (multiplicity of infection)?arrow_forwardDraw and label the E. coli host cell/T4 bacteriophage interaction.?arrow_forwardWhy are prokaryotes able to replicate so much more quickly than eukaryotes? What cellular components must be copied before a cell splits in two? Why is this difference in replication rate an important part of our vulnerability to bacterial pathogens?arrow_forward
- After a phage injects its DNA into a bacterial cell, the cell begins making proteins that make up the phage coat. Why does the same thing not happen when a generalized transducing particle injects the DNA it carries?arrow_forwardWhat advantages might a phage gain by being capable of lysogeny?arrow_forwardYou are studying your favorite (hypothetical) bacteriophage named M277. It infects E. coli and stays dormant within the bacterial cell. How does a phage become dormant within a cell? Generally, describe the steps of infection by phage in this scenarioarrow_forward
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