ESSENTIALS OF GENETICS-MODIFIED ACCESS
9th Edition
ISBN: 9780134190006
Author: KLUG
Publisher: PEARSON
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Chapter 8, Problem 10PDQ
The bacteriophage genome consists primarily of genes encoding proteins that make up the head, collar and tail, and tail fibers. When these genes are transcribed following phage infection, how are these proteins synthesized, since the phage genome lacks genes essential to ribosome structure?
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The bacteriophage genome consists primarily of genes encodingproteins that make up the head, collar and tail, and tail fibers When these genes are transcribed following phage infection, howare these proteins synthesized, since the phage genome lacksgenes essential to ribosome structure?
When bacteriophage P1 causes E. coli to lyse, the resulting materialis called a P1 lysate. What type of genetic material would befound in most of the P1 phages in the lysate? What kind of geneticmaterial is occasionally found within a P1 phage?
One of the reasons why phage therapy has not been applied widely is that bacteria can become resistant to bacteriophages as well, through mutations in genes encoding for specific proteins. What would be a protein in the bacterial cell that, if mutated, would make that cell resistant to phage infection?
Chapter 8 Solutions
ESSENTIALS OF GENETICS-MODIFIED ACCESS
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- What is meant by the term site-specific recombination as used in identifying the processes that lead to the integration of temperate bacteriophages into host bacterial chromosomes during lysogeny or to the formation of specialized transducing phage?arrow_forwardwhen various strains of lambda phage are seeded on a lawn of e.coli, they can form clear or turbid plaques. Explain the difference between the two types of plaques. can all bacteriophage form clear and turbid plaques?arrow_forwardDiscuss the genetic switch that controls the lytic versus lysogenic path in bacteriophage lambda.arrow_forward
- Why is formation of the replicative form of fX174 necessary inorder to make phage-specific mRNA?arrow_forwardWhat would happen to the ability of bacteriophage λ tolyse a host cell if it acquired a mutation in the OR bindingsite for the Cro protein? Why?arrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forward
- What is the toxin produced by E. coli O157:H7 that was encode by phage as a consequence of lysogenic conversion? Explain the medical importance.arrow_forwardDoes the Hershey and Chase experiment rule out the possibility that RNA is the genetic material of T2 phage? Explain. If it does not, redesign the experiments of Hershey and Chase to distinguish between DNA and RNA in the T2 phage.arrow_forwardIn the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.arrow_forward
- What structures on bacterial host cells serve to allow bacteriophage to recognize them? What component of these structures may be most readily recognizable to bacteriophages?arrow_forwardWhat evolutionary advantage would a retrovirus gain by having the ability to regulate the sites of splicing of its RNA?arrow_forwardSome S. aureus strains can produce an enzyme called beta-lactamase, which breaks down beta-lactam drugs like methicillin. These strains that make beta-lactamase have a gene called mecA. Draw a diagram of the bacterial DNA encoding this gene, including the following terms: promoter, terminator, coding region.arrow_forward
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