Microbiology with Diseases by Taxonomy (5th Edition)
5th Edition
ISBN: 9780134019192
Author: Robert W. Bauman Ph.D.
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 1VI
Label the reagents and steps of PCR on the figure below. Indicate the temperature of the reaction at each numbered step.
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The laboratory has lost the protocol to follow during the PCR reaction and only give you this. Please fill in the blanks:
94°C _______________ step for _______ minutes
52°C _______________ step for _______ seconds
72°C________________ step for ______ minutes 72°C _______________ step for ______ minutes
Why different temperatures are used in a PCR reaction? Please answer at your own words.
In a typical PCR reaction, describe what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.
Chapter 8 Solutions
Microbiology with Diseases by Taxonomy (5th Edition)
Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MCCh. 8 - Which of the following would be most useful in...Ch. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MC
Ch. 8 - Prob. 1TFCh. 8 - Prob. 2TFCh. 8 - Prob. 3TFCh. 8 - ________ Protoplast fusion is often used in the...Ch. 8 - Prob. 5TFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - Prob. 2CTCh. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - How is the result of a Southern blot similar to...Ch. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - If a gene contains the sequence TACMTCGCATTGM,...Ch. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Why did the discovery and development of...Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Why dont scientists who work with recombinant DNA...
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- Name four reagents used during a PCR reactionarrow_forwardA thermocycler is a machine used to heat and cool the PCR sample in a series of three steps per cycle. Describe briefly what happens in each step. a. Step 1 - Heat briefly (for 1 min) to 94 degrees Celsius to ___________ b. Step 2 - Cool (for 1 min) to 50 degrees Celsius to _____________ c. Step 3 - Heat (for 1 min) to 72 degrees Celsius to _____________arrow_forwardList the steps of a PCR cyclearrow_forward
- If you wanted to prepare only one PCR reaction, how much of each reagent would you add to the PCR tube?arrow_forwardA hand-drawn PCR diagram to show the role of each component and relevance of each temperature shift during the PCR reaction. Scan your figure, and embed the scan within your report.arrow_forwardWhich of the following ARE part of a typical PCR reaction mixture? DNA ligase dNTPs (mix of nucleoside tri-phosphates) RNA primers made by primase enzyme template DNA, often from cells collected from hair, cheek swab, or blood 2 DNA primers polymerase enzymearrow_forward
- Identify if the following statements are true or false. Quantitative PCR is also known as the real-time PCR Real-time PCR method uses fluorescent dyes such as acridine orangearrow_forwardIn a typical PCR reaction what is happening in stages occurring at temperature ranges (a) 92–95°C, (b) 45–65°C, and (c) 65–75°C.arrow_forwardIdentify three items (aside from water) that must be used in a Polymerase Chain Reaction (PCR) mixture.arrow_forward
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