Prescott's Microbiology
11th Edition
ISBN: 9781260211887
Author: WILLEY, Sandman, Wood
Publisher: McGraw Hill
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Textbook Question
Chapter 8, Problem 3AL
Suppose you tested the effectiveness of disinfectants with the phenol coefficient test and obtained the results shown in the “Bacterial Growth After Treatment” table. What disinfectant can you safely say is the most effective? Can you determine its phenol coefficient from these results?
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Students have asked these similar questions
A disk-diffusion test using E. coli gave the following results:
Disinfectant Zone of inhibition (diameter in mm)
A 15
B 2.0
C 10
D 5
In the table above, which compound was the most effective against E. coli?
A) A
B) B
C) C
D) D
E) The answer cannot be determined based on the information provided.
Explain why only gram-negative cells undergo decolorization during the gram staining procedure.
Cite the purpose of each of the following reagents in a differential staining procedure.
Primary stain
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Decolorizing Agent
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What might happen if the Gram staining procedure is performed on a culture incubated for a little over a day
EXPERIMENT : REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD
Objective
To learn on the technique of freeze drying for microbial sample preparation and preservation.
Result
Weight of test tube (g) -Wi
15.34
Weight of test tube with cells (g) - Ww
34.65
Amount of water removal
= weight of test tube with cells – weight of test tube
= 34.65 g – 15.34 g
= 21.16 g
QUESTION: Discuss the result and explain
the freeze drying method that used in this experiment
how the freeze drying method
Chapter 8 Solutions
Prescott's Microbiology
Ch. 8.1 - MICRO INQUIRY Which types of agents can be used...Ch. 8.1 - Retrieve, Infer, Apply 1. Define the following...Ch. 8.1 - Retrieve, Infer, Apply 2. What is the difference...Ch. 8.2 - MICRO INQUIRY Examine graph (a). How long would it...Ch. 8.3 - MICRO INQUIRY How might one verify that filtration...Ch. 8.3 - Retrieve, Infer, Apply 1. What are depth filters...Ch. 8.3 - Retrieve, Infer, Apply 2. Describe the operation...Ch. 8.4 - Retrieve, Infer, Apply 1. Describe how an...Ch. 8.4 - Retrieve, Infer, Apply 2. In the past, spoiled...Ch. 8.4 - Prob. 3CC
Ch. 8.4 - Retrieve, Infer, Apply 2. What is the correlation...Ch. 8.5 - MICRO INQUIRY Why is it important that all of...Ch. 8.5 - MICRO INQUIRY Why are cross-linking agents such as...Ch. 8.5 - Retrieve, Infer, Apply 1. Why are most...Ch. 8.5 - Retrieve, Infer, Apply Construct a table that...Ch. 8.5 - Retrieve, Infer, Apply 3. Which disinfectants or...Ch. 8.5 - Retrieve, Infer, Apply 4. How do phenolic agents...Ch. 8.5 - Retrieve, Infer, Apply 5. Which physical or...Ch. 8.6 - Retrieve, Infer, Apply 1. Briefly explain how the...Ch. 8.6 - Retrieve, Infer, Apply How does being in a biofilm...Ch. 8.6 - Retrieve, Infer, Apply Suppose hospital custodians...Ch. 8.6 - Retrieve, Infer, Apply Briefly describe the phenol...Ch. 8.6 - Retrieve, Infer, Apply Why might it be necessary...Ch. 8.7 - Retrieve, Infer, Apply How would you explain to a...Ch. 8.7 - Retrieve, Infer, Apply Propose the use of specific...Ch. 8 - Prob. 1RCCh. 8 - Throughout history, spices have been used as...Ch. 8 - Design an experiment to determine whether an...Ch. 8 - Suppose you tested the effectiveness of...Ch. 8 - Prob. 4AL
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- EXPERIMENT : REMOVAL OF WATER FROM MICROBIAL CELLS USING FREEZE DRYING METHOD Objective To learn on the technique of freeze drying for microbial sample preparation and preservation. Result Weight of test tube (g) -Wi 15.34 Weight of test tube with cells (g) - Ww 34.65 Amount of water removal = weight of test tube with cells – weight of test tube = 34.65 g – 15.34 g = 21.16 g QUESTION: Discuss the result and explain the freeze drying method that used in this experiment.arrow_forwardCompare and contrast use dilution and disk diffusion methods of evaluating disinfectants. Please explain phenol coefficient.arrow_forwardAnswer the ff with not more than two sentences: Why should Erlenmeyer flasks hold not more than 60% of their capacity? When can gelatin be used in culture media preparation? How does cotton plug prevent contamination of the medium? Why should caps of glassware be loosened first before sterilization?arrow_forward
- Suppose you are to observe an unknown bacterial slide. In your initial observation, it appears to be in fading color and the shape of the specimen seems to be diffused. How are you going to troubleshoot the focusing of the specimen for you to identify clearly the bacteria?arrow_forwardOf what practical importance are air borne microorganisms to the laboratory workers? What precautions should be taken to control laboratory contaminants? Why are petri dishes incubated in an inverted position? Of what advantage is the using a solid and a liquid medium?arrow_forwardgive one reason why is it necessary to keep stirring the agar medium mixture in a beaker when heating in a hotplate?arrow_forward
- If you have a culture with 1 x 106 cells per ml, how could you use serial dilutions to obtain a suspension with 5 x 102 cells per ml? Show your answer using a serial dilution scheme or diagramarrow_forwardIf you diluted 200 uL of your culture into 1800 uL of water what is your dilution factor? (Show your work)arrow_forwardYou are asked to make a 1:1000 dilution of a bacterial culture in a final volume of 5000 mL. What should be your initial volume from the original culture? Show solution.arrow_forward
- Compare and contrast antiseptic and disinfectant. What is the hazard of the splattering tendency in flame sterilizing an inoculating loop? Can inoculation loops and needles be sterilized by autoclaving? Explain.arrow_forwardUsing your fingers, you are asked to aseptically touch the surface of a sterile agar plate. Illustrate the possible result from this step if your fingers are (a) unwashed – touched various things prior to placing on agar surface, and (b) washed with soap or disinfected with 70% alcohol. Describe the relative abundance of microbial growth observed on the plates. List and draw the possible characteristics of an isolated bacterial colony that can be observed based on type of (a) margin, (b) elevation, (c) texture, and (d) optical property.arrow_forwardCan culture media be sterilized in the hot air sterilizer? Why or why not?arrow_forward
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