MICROBIOLOGY W/ACCESS
4th Edition
ISBN: 9781266808685
Author: Cowan
Publisher: MCG
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Chapter 8, Problem 3CTQ
Summary Introduction
To determine:
The reason that the discovery of thermophilicarchaea and their associated DNA polymerase was critical to the successof PCR technology.
Introduction:
The polymerase chain reaction is a technique to amplify a piece of DNA rapidly outside a living cell. It uses the same mechanism for multiplication of the DNA as the cells use for
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Chapter 8 Solutions
MICROBIOLOGY W/ACCESS
Ch. 8.1 - Describe the relationship among metabolism,...Ch. 8.1 - Fully discuss the structure and function of...Ch. 8.1 - Differentiate between an apoenzyme and a...Ch. 8.1 - Differentiate between an endoenzyme and an...Ch. 8.1 - Diagram the four major patterns of metabolism.Ch. 8.1 - Describe how enzymes are controlled.Ch. 8.2 - Name the chemical in which energy is stored in...Ch. 8.2 - Create a general diagram of a redox reaction.Ch. 8.2 - Identify electron carriers used by cells.Ch. 8.3 - Prob. 2CF
Ch. 8.3 - List three basic catabolic pathways and the...Ch. 8.3 - Construct a paragraph summarizing glycolysis.Ch. 8.3 - Describe the Krebs cycle and compare the process...Ch. 8.3 - Discuss the significance of the electron transport...Ch. 8.3 - State two ways in which anaerobic respiration...Ch. 8.3 - Summarize the steps of microbial fermentation and...Ch. 8.3 - Prob. 16AYPCh. 8.4 - Prob. 17AYPCh. 8.4 - Define amphibolism.Ch. 8.5 - Summarize the overall process of photosynthesis in...Ch. 8.5 - Discuss the relationship between light-dependent...Ch. 8.5 - Explain the role of the Calvin cycle in the...Ch. 8 - Prob. 1CFCh. 8 - Prob. 1MCQCh. 8 - An enzyme a. becomes part of the final products....Ch. 8 - An apoenzyme is where the ____ is located. a....Ch. 8 - Many coenzymes are a. metals. b. vitamins. c....Ch. 8 - Prob. 5MCQCh. 8 - Energy is carried from catabolic to anabolic...Ch. 8 - A product or products of glycolysis is/are a. ATP....Ch. 8 - Prob. 8MCQCh. 8 - Complete oxidation of glucose in aerobic...Ch. 8 - ATP synthase complexes can generate ______ ATP(s)...Ch. 8 - True-False Questions. If the statement is true,...Ch. 8 - An enzyme lowers the activation energy required...Ch. 8 - One cycle of fermentation yields more energy than...Ch. 8 - Energy in biological systems is primarily...Ch. 8 - Exoenzymes are produced outside the cell.Ch. 8 - Prob. 1CTQCh. 8 - Prob. 2CTQCh. 8 - Prob. 3CTQCh. 8 - Prob. 4CTQCh. 8 - Prob. 5CTQCh. 8 - Prob. 6CTQCh. 8 - Prob. 7CTQCh. 8 - Prob. 8CTQCh. 8 - Prob. 9CTQCh. 8 - Provide evidence in support of or refuting the...Ch. 8 - Prob. 1CCCh. 8 - Prob. 2CCCh. 8 - Prob. 3CCCh. 8 - From chapter 4, figure 4.16. On the enlarged...Ch. 8 - Using the words that follow, please create a...
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- Due to climate change, food security is highly compromised. Sequentially explain recombinant DNA techniques you would use to isolate and transfer a heat resistant gene from a bacteria to help maize plants thrive in areas with water scarcity.arrow_forwardWhy is it important to use a hyperthermophilic DNA polymerase in PCR? a) Because only hyperthermophiles have DNA polymerases. b) Because hyperthermophilic DNA polymerase is able to resist the saline reaction conditions. c) Because hyperthermophilic DNA polymerase is faster than other polymerases. d) Because hyperthermophilic DNA polymerase is able to resist denaturation at 95℃.arrow_forwardFrederick Griffith documented bacterial transformation. Which statement is true concerning his experiments? DNA that was not contained within a live cell caused the death of the host organism. DNA from dead Streptococcus cells entered live cells and altered the live cell's characteristics. The two strains of Streptococcus were visually indistinguishable. Heat treatment of the Streptococcus strains destroyed the DNA molecules.arrow_forward
- You got an opportunity to join a professor lab who is working in-vivo model and specifically looking at the dysregulation of mitochondria in liver. He asked you to isolate mitochondria from a Rat liver and placed in an assay medium. Based on the knowledge you gain in this course so far, please answer the following questions: a) Which technique will you use to isolate mitochondria? b) What happens to the pH of the medium when the medium is kept anaerobic? c) What happens when O2-saturated saline is added to the mixture?arrow_forwardLike eukaryotes, bacteria and archaea enclose their DNA in a nucleus.arrow_forwardChoose the one answer that fits best. Which of the following statements regarding bacteria is NOT correct? a. During conjugation, two bacteria exchange genetic information directly via pili b. Bacterial populations have a higher mutation rate because their DNA Polymerases do not have proofreading capability c. Bacterial growth by binary fission is exponential and each division usually takes only a couple of hour or less d. Plasmids carry just a few genes and are replicated independently from the bacterial chromosome e. Cocci that are arranged in clusters/bunches are called staphylococciarrow_forward
- Using what you now know about restriction enzymes, why do you suppose that wells in the gel contained more than one band?arrow_forwardThe result Jake obtained after transforming his competent Escherichia coli cells with plasmids is as shown below: Is the transformation experiment conducted a success? Justify your answer.arrow_forwardIn order to transform a bacteria, the cell wall should be perforated either through physical or chemical means. true or false?arrow_forward
- This research utilized a modified method of preparing activated carbons (ACs) derived from waste coconut shells loaded with silver nanoparticles (AgNP). AC was prepared through chemical impregnation method of coconut shells in H3PO4 and carbonization in a muffle furnace, while AgNP was prepared following the Turkevich method. These two substances were then combined to form silver nanoparticle-loaded activated carbon (AgAC). The samples were subjected to different characterization techniques: FTIR analysis to test the purity of the AC, FE-SEM to analyze the surface morphology of AgAC, and UV-Vis determination of AgNP formation. After characterization, the antibacterial activity of AgAC was evaluated using the Agar Well Diffusion Method. Results show that AgNPs were observed to form at an average maximum absorbance of 2.063 at a wavelength interval of 421-423 nm. It was also observed that the synthesized AC exhibited the FTIR result comparable to the commercially-available AC. Further,…arrow_forwardConjugation is the uptake of DNA from the environment. true or falsearrow_forwardGene cloning is a process in which a gene of interest is identified and made into multiple copies. Illustrate the steps in gene cloning using Escherichia coli as the host cell after PCR has been carried out to amplify a gene of interest.arrow_forward
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