Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Chapter 8, Problem 3MC
Penicillin enrichment of mutants works on the principle that the antibiotic
a) kills only Gram-positive cells.
b) primarily kills cells during the
c) kills most Gram-negative cells.
d) kills only growing cells.
e) inhibits formation of the lipopolysaccharide layer.
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In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium?
Results from a Kirby Bauer antibiotic assay on a Gram-negative bacterial culture are described as follows: A) the bacterium is resistant to penicillin, an antibiotic that targets synthesis of the peptidoglycan cell wall and B) the bacterium is resistant to tetracycline, an antibiotic that targets the small subunit of the ribosome, inhibiting protein synthesis. Which of the results represents intrinsic resistant and which represents acquired resistants?
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Chapter 8 Solutions
Nester's Microbiology: A Human Perspective
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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- What would happen if recombinant microbes could transfer genetically altered genes into other microorganisms for which they were not originally intended?arrow_forwardIn Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardFrom what other environments might E. coli bacteriophages be readily isolated?arrow_forward
- Does bacterial transformation occur naturally?arrow_forwarda bacteriophage has been isolated from the growth chambers that has evolved the capability to the methylate its own DNA when it is replicated inside the host bacterium. why is this a problem?arrow_forwardHow do we know that intergenic exchange occurs in bacteriophages?arrow_forward
- You have isolated a strain of E.coli that is resistant to penicillin,streptomycin,chloramphenicol and tetracycline. You also observe that when you mix this strain with a strain that is sensitive to all four antibiotics, the new strain becomes resistant to streptomycin,penicillin and chloramphenicol but not tetracycline. Explain how this is so?arrow_forwarddescribe a semi-synthetic antibioticarrow_forwardIn the plaque assay, what is the precise origin of a single plaque?arrow_forward
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