Nester's Microbiology: A Human Perspective
9th Edition
ISBN: 9781259709999
Author: Denise G. Anderson Lecturer, Sarah Salm, Deborah Allen
Publisher: McGraw-Hill Education
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Textbook Question
Chapter 8, Problem 5MC
You are trying to isolate a mutant of wild-type E. coli that requires histidine for growth. This can best be done using
1)direct selection.
2)replica plating.
3)penicillin enrichment.
4)the Ames test.
5)reversion.
a) 1, 2
b) 2, 3
c) 3, 4
d) 4, 5
e) 1, 5
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In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium?
In the bacterial transformation experiment, what is the primary purpose of using an ampicillin-containing medium?
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Chapter 8 Solutions
Nester's Microbiology: A Human Perspective
Ch. 8 - Prob. 1SACh. 8 - Why is deleting one nucleotide generally more...Ch. 8 -
3. What type of mutation in an operon is most...Ch. 8 -
4. What is meant by "proofreading" with respect...Ch. 8 - Why would a cell use SOS repair, considering that...Ch. 8 - Why is replica plating used to isolate an...Ch. 8 - What is transduction?Ch. 8 -
8. How is an F+ strain different from an Hfr...Ch. 8 - Name four mobile genetic elements.Ch. 8 - Why are R plasmids important?
Ch. 8 - UV light exposure forms a) covalent bonds between...Ch. 8 -
2. If cells were exposed to UV light, the highest...Ch. 8 -
3. Penicillin enrichment of mutants works on the...Ch. 8 -
4. Mechanisms that repair errors in nucleotide...Ch. 8 -
5. You are trying to isolate a mutant of...Ch. 8 -
6. All plasmids
1) carry genes for antimicrobial...Ch. 8 -
7. Adding DNase to a mixture of donor and...Ch. 8 -
8. An F pilus or its equivalent is essential...Ch. 8 - A plasmid that can replicate in E. coli and...Ch. 8 -
10. The frequency of transfer of an F' molecule...Ch. 8 -
1. Some bacteria may have higher mutation rates...Ch. 8 -
2. A pharmaceutical researcher is disturbed to...Ch. 8 - Prob. 1CTCh. 8 -
2. You have isolated a strain of E. coli that is...
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- Petri dish. Explain in your own words how bacteria are grown to form the sample for the antibiotic resistance test?arrow_forwardExplain The replica plating technique?arrow_forwardWhich cultures would be suitable for the transduction experiment and why? Which cultures would not be suitable for the transduction experiment and why?arrow_forward
- What is the point of doing a modified Furter-Meyer Test? What is the underlying idea of this test? How did you figure out that this test had a positive result?arrow_forwardExplain the bacterial transformation experiments of Avery and his colleagues and the results they obtained?arrow_forwardWhat is the purpose of the S-9 in the Ames test?arrow_forward
- At what stage of the culture should bacterial colonies be harvested for plasmid DNA extraction? How about for genomic DNA extraction? What distinguishes the xanthogenate-based methodology from the traditional phenol/chloroform method for isolating DNA from bacteria? What makes potassium ethyl xanthogenate efficient in isolating DNA from a variety of microorganisms?arrow_forwardDetermining the genetic profile of a bacterium for its identification is a part of which step of the identification process?arrow_forwardBacterial mutants can be classified on the basis of the method, Which is those methods?arrow_forward
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