Essentials of Genetics Plus Mastering Genetics with eText -- Access Card Package (9th Edition) (Klug et al. Genetics Series)
9th Edition
ISBN: 9780134047201
Author: William S. Klug, Michael R. Cummings, Charlotte A. Spencer, Michael A. Palladino
Publisher: PEARSON
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Textbook Question
Chapter 8, Problem 4PDQ
With respect to F+ and F- bacterial matings,
(a) How was it established that physical contact was necessary?
(b) How was it established that chromosome transfer was unidirectional?
(c) What is the genetic basis of a bacterium being F+?
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With respect to F+ and F- bacterial matings, answer the following questions: (a) How was it established that physical contact between cells was necessary? (b) How was it established that chromosome transfer was unidirectional? (c) What is the genetic basis for a bacterium’s being F+?
Which bacterial genetic transfer process does not require recombinationwith the bacterial chromosome?
When the interrupted mating technique was used withfive different strains of Hfr bacteria, the following orders ofgene entry and recombination were observed. On the basisof these data, draw a map of the bacterial chromosome.Do the data support the concept of circularity?HfrStrain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A B
Chapter 8 Solutions
Essentials of Genetics Plus Mastering Genetics with eText -- Access Card Package (9th Edition) (Klug et al. Genetics Series)
Ch. 8 -
CASE STUDY | To treat or not to treat
A...Ch. 8 - CASE STUDY | To treat or not to treat A...Ch. 8 - Prob. 3CSCh. 8 - Prob. 4CSCh. 8 - HOW DO WE KNOW? In this chapter, we have focused...Ch. 8 -
CONCEPT QUESTION
2. Review the Chapter Concepts...Ch. 8 -
3. Distinguish among the three modes of...Ch. 8 - With respect to F+ and F- bacterial matings, (a)...Ch. 8 - List all of the differences between F+ × F– and...Ch. 8 - Describe the basis for chromosome mapping in the...
Ch. 8 - Why are the recombinants produced from an Hfr × F–...Ch. 8 - Describe the origin of F' bacteria and...Ch. 8 -
9. Describe the mechanism of transformation.
Ch. 8 - .
10. The bacteriophage genome consists primarily...Ch. 8 - Prob. 11PDQCh. 8 - In the plaque assay, what is the precise origin of...Ch. 8 -
13. In the plaque assay, exactly what makes up a...Ch. 8 - A plaque assay is performed beginning with 1.0 mL...Ch. 8 -
15. Describe the difference between the lytic...Ch. 8 - Prob. 16PDQCh. 8 -
17. Explain the observations that led Zinder and...Ch. 8 -
18. Describe the execution of and rationale...Ch. 8 - If a single bacteriophage infects one E. coli cell...Ch. 8 - A phage-infected bacterial culture was subjected...
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- When the interrupted mating technique was used with five different strains of Hfr bacteria, the following orders of gene entry and recombination were observed. On the basis of these data, draw a map of the bacterial chromosome. Do the data support the concept of circularity?Hfr Strain Order1 T C H R O2 H R O M B3 M O R H C4 M B A K T5 C T K A Barrow_forwardHow many base pairs are in a molecule of phage T2 DNA that is52 μm long?arrow_forwardWhat is horizontal gene transfer? What are the three mechanisms for this to occur in bacteria? What are the components needed for the processes of transformation, conjugation, and transduction? How does each process occur? What genes are involved in each process? How do generalized and specialized transduction differ? What is the end result of each? What is recombination? What is the importance to bacteria & archaea? What are the two types of recombination? What are the details of each type? What components are needed for each type?arrow_forward
- In the Hershey Chase experiment, why was the localization of radio-labeled phage of macromolecules in the cytoplasm the reason that the genetic material identity was proved.arrow_forwardIn Figure 5-2, in which of the four processes shown can acomplete bacterial genome be transferred from one cellto another?arrow_forwardWhat is recombination? What is the importance to bacteria & archaea? What are the two types of recombination? What are the details of each type? What components are needed for each type?arrow_forward
- What structures on bacterial host cells serve to allow bacteriophage to recognize them? What component of these structures may be most readily recognizable to bacteriophages?arrow_forwardDoes bacterial transformation occur in nature?arrow_forwardIn what ways does conjugation create bacterial cells that are at least partially diploid? Is this a stable condition?arrow_forward
- In Hershey-Chase experiment, bacteriophages protein coats were tagged with radioactive isotope S-32. These phages were used to infect E. coli cells and the cells were further centrifuged to form pellets. Why was the radioactivity level of S-32 found greater outside the cells compared to the E. coli cell pellets? Explain briefly. If the experiment is repeated in the same manner but this time the phage protein coats are labelled with isotope X and the phage DNA with isotope Y, which isotope’s radioactivity will be found in greater amounts in the E. coli cell pellets after centrifugation? Explain briefly.arrow_forwardWhat is meant by the term site-specific recombination as used in identifying the processes that lead to the integration of temperate bacteriophages into host bacterial chromosomes during lysogeny or to the formation of specialized transducing phage?arrow_forwardWhat is replica plating?arrow_forward
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genetic recombination strategies of bacteria CONJUGATION, TRANSDUCTION AND TRANSFORMATION; Author: Scientist Cindy;https://www.youtube.com/watch?v=_Va8FZJEl9A;License: Standard youtube license