Microbiology: With Diseases by Taxonomy - With Lab. Man
5th Edition
ISBN: 9780134589824
Author: BAUMAN
Publisher: PEARSON
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Chapter 8, Problem 5CT
Summary Introduction
To describe:
The fragments obtained by digesting the enzyme
Introduction:
Restriction digestion takes place at specific sites in the genome. It depends upon the type of restriction enzymes used for digesting the palindromic repeat sequences in
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Chapter 8 Solutions
Microbiology: With Diseases by Taxonomy - With Lab. Man
Ch. 8 - Which of the following statements is true...Ch. 8 - A DNA gene synthesized from an RNA template is...Ch. 8 - Prob. 3MCCh. 8 - Prob. 4MCCh. 8 - Prob. 5MCCh. 8 - Which of the following would be most useful in...Ch. 8 - Prob. 7MCCh. 8 - Prob. 8MCCh. 8 - Prob. 9MCCh. 8 - Prob. 10MC
Ch. 8 - Prob. 1TFCh. 8 - Prob. 2TFCh. 8 - Prob. 3TFCh. 8 - ________ Protoplast fusion is often used in the...Ch. 8 - Prob. 5TFCh. 8 - Label the reagents and steps of PCR on the figure...Ch. 8 - Describe three artificial methods of introducing...Ch. 8 - Prob. 2SACh. 8 - Prob. 3SACh. 8 - Prob. 4SACh. 8 - List three potential problems of recombinant DNA...Ch. 8 - Examine the restriction sites listed in Table 8.1...Ch. 8 - Prob. 2CTCh. 8 - A thermocycler uses DNA polymerase from...Ch. 8 - How is the result of a Southern blot similar to...Ch. 8 - Prob. 5CTCh. 8 - Prob. 6CTCh. 8 - If a gene contains the sequence TACMTCGCATTGM,...Ch. 8 - Prob. 8CTCh. 8 - Prob. 9CTCh. 8 - Using the following terms, fill in the following...Ch. 8 - Why arent the terms recombinant DNA technology...Ch. 8 - Why did the discovery and development of...Ch. 8 - Why wasnt polymerase chain reaction (PCR)...Ch. 8 - Why dont doctors routinely insert genes into their...Ch. 8 - Why dont scientists who work with recombinant DNA...
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- Enzymes isolated from bacteria that cut DNA at specificsequences are called ____________________.arrow_forwardIs there any situation in which DNA is made based on a RNA template? What is the enzyme involved?arrow_forwardA DNA probe with sequence TCAGGCTTCAG would bind most strongly to which of the following DNA fragments? a. AGTCCGAAGTC c. GACTTCGGACT b. TCAGGCTTCAG d. UGAGGCUUGAGarrow_forward
- The following DNAs were incubated with E. coli DNA Polymerase I and dNTPs. Write the products expected in each reaction. Indicate the activity used in each reaction.arrow_forwardA microbiologist discovers a new type II restriction endonuclease. When DNA is digested by this enzyme, fragments that average 1,048,500 bp in length are produced. What is the most likely number of base pairs in the recognition sequence of this enzyme?arrow_forwardWithin the template strand of the DNA in a gene, the sequence TAT is changed by mutation to TAC. Following replication of the DNA, what change in the polypeptide product would this cause?arrow_forward
- Define and indicate the significance of DNA ligasearrow_forwardWhen circular DNA is sequenced, the nucleotide base pairs are numbered starting from a fixed position on the DNA, all the way around, usually in a clockwise manner. a DNA molecule that is 3133 base pairs long is digested with RsaI restriction enzyme recognition sites at base numbers 366, 1534, and 2207. What are the sizes of the DNA fragments that will be produced after the DNA is digested with RsaI?arrow_forwardThe enzymes DNA polymerase in E.coli is a DNA dependent polymerace and also has the ability to proof-read the DNA strand being synthesised?arrow_forward
- Several temperature-sensitive mutant strains of E. coli displaythe following characteristics. Predict what enzyme or function isbeing affected by each mutation. Supercoiled strands remain after replication, which is never completed.arrow_forwardName the enzymes that cut the DNA at specific sequences and create sticky ends.arrow_forwardIdentify the dinucleotide CA repeat region and the score in the following sequence:TGGCACACTCACACCACACAGACAGTTAarrow_forward
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